Team:UNIPV-Pavia/Notebook/Week3Sep

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m (→Week from September 14th, to September 20th, 2009)
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Revision as of 09:12, 20 October 2009

EthanolPVanimation.gif

December 2008
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March 2009
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April 2009
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May 2009
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June 2009
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July 2009
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August 2009
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September 2009
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October 2009
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Week from September 14th, to September 20th, 2009

Previous Week Next Week

September, 14th

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September, 15th

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September, 16th

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September, 17th

pH sensor
  • Infection of 4 ml LB + Amp with 8 ul from glycerol stock of:
    • RBS33
    • A2
    • K116002 (from now on it will be indicated only as pNhaA)
  • Overnight incubation of these three cultures at 37°C, 220 rpm.


Top

September, 18th

pH sensor

1st experiment

  • We put 50 ul into 5 ml LBK + Amp pH 6,6 falcon for each of the overnight cultures RBS33 - A2 - pNhaA and incubated them for four hours and a half at 37°C, 220 rpm.
  • OD measure of the three falcons with TECAN in order to make dilutions with a final volume of 3 ml and an OD equal to 0,02.
  • We diluted:
    • RBS into:
      • LBK + Amp pH 5,5
      • LBK + Amp pH 6,6
      • LBK + Amp pH 7,5
      • LBK + Amp pH 8,5
    • pNhaA into:
      • LBK + Amp pH 5,5
      • LBK + Amp pH 6,6
      • LBK + Amp pH 7,5
      • LBK + Amp pH 8,5
    • A2 into:
      • LBK + Amp pH 5,5
      • LBK + Amp pH 6,6
      • LBK + Amp pH 7,5
      • LBK + Amp pH 8,5
  • We started this 6 hours' experiment that performed absorbance and fluorescence measures (on three different wells) for each sample every 5 minutes.


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September, 19th

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September, 20th

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