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Revision as of 12:01, 20 October 2009
Materials
- Backbone Plasmids
- Plasmid pSB3C5 with [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] : 2009 Kit Plate 1 [5C]
- Plasmid pSB3T5 with [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] : 2009 Kit Plate 1 [9C]
- Promoters
- Promoter arsR [ParsR], zntA [Pznt] and yodA [PyodA](ref) originated from genomic DNA of Escherichia coli XL-1 Blue
- Protein Coding Sequences
- [http://partsregistry.org/Part:BBa_E0044 Green fluorescent protein(BBa_E0044)] : 2009 Kit Plate 1 [14G]
- [http://partsregistry.org/Part:BBa_E1010 Red fluorescent protein(BBa_E1010)] : 2009 Kit Plate 1 [18F]
- Aryl acylamidase protein(ref) : [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=Nucleotide&list_uids=227462445&dopt=GenBank new biological part]
- Primers
| Template
| Primer
| Sequence
| Length(bp)
|
| Plasmid pSB3C5 | pSB3C5_F | gctgctgttTAATAAtactagtagcggccgctgc | 34
|
| pSB3C5(+Pars)_R | taataggtgtgaattttgagttggCtctagaagcggccgcga | 42
|
| Plasmid pSB3T5 | pSB3T5_F | gacgaaaactacgctgctgctgttTAATAAtactagtagcggccgctgc | 49
|
| pSB3T5_R | GTCGGCAATATGAAGctctagaagcggccgcga | 33
|
| Promoter yodA | PyodA_F | cggccgcttctagagCTTCATATTGCCGACAAAGTACG | 38
|
| PyodA_R | ATGTGACTTACCCATaacAGTTTCCTCCAGAGTCATTG | 38
|
| Green fluorescent protein | GR(+Pars)_F | aattcacacctattaccttcctctgcacttacacattcgttaagtcatatATGc | 78
|
| gtaaaggagaagaacttttcactg
|
| GR(+Pznt)_R | ctggagtcgactccagagtcaagttttatcagagatacagcgagcggacg | 84
|
| TCTAGTttattaaacagcagcagcgtagttttcg
|
| Red fluorescent protein
| RF(+Pznt)_F | tgataaaacttgactctggagtcgactccagagtgtatccttcggttaatATG | 75
|
| gcttcctccgaagacgttatca
|
| RF(+AAV tag)_R | TTATTAaacagcagcagcgtagttttcgtcgtttgctgcAgcaccggtgg | 59
|
| agtgacgac
|
| Aryl acylamidase
| AMD_F | CTGGAGGAAACTGTTatgGGTAAGTCACATTCGCCAG | 37
|
| AMD(+AAV tag)_R | TTATTAaacagcagcagcgtagttttcgtcgtttgctgcCAGGGGC | 55
|
| CGTCCGGCG
|
- Chemicals
- Acetaminophen (Sigma)
- CdCl2∙H2O (Junsei)
- ZnCl2 (Sigma)
- KH2AsO3 (Sigma)
- Luria-Bertani broth & Bacto agar (Difco)
- o-Cresol (Sigma)
- CuSO4∙5H2O (Sigma)
|
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