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Team:TorontoMaRSDiscovery/11 August 2009
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(New page: =August 11, 2009= #Digested 4, 5, Enc, C(E+P), C(X+S) #*BB4 did not digest #*5, C is stored in fridge #Calvin suggested increasing the concentration of enzyme and increase time of digestio...)
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(New page: =August 11, 2009= #Digested 4, 5, Enc, C(E+P), C(X+S) #*BB4 did not digest #*5, C is stored in fridge #Calvin suggested increasing the concentration of enzyme and increase time of digestio...)
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Revision as of 15:12, 20 October 2009
August 11, 2009
- Digested 4, 5, Enc, C(E+P), C(X+S)
- BB4 did not digest
- 5, C is stored in fridge
- Calvin suggested increasing the concentration of enzyme and increase time of digestion due to thawing enzymes over weekend in fridge
- Miniprepped 1+2(1) with new kit, got great yield ~100ng/ul
- Gel extracted C (X+S) after it was CIPed
- Overnight ligation of Enc+C in PCR machine
- Calvin gave us 2 vials of RbCl cells in -80C
- one time use only
- thaw and add DNA
