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Team:EPF-Lausanne/Protocols/Illumination
From 2009.igem.org
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'''Material''' | '''Material''' | ||
| - | *LED blue 3mm (Distrelec) | + | *LED blue (458nm) 3mm (Distrelec ref number: 63 12 48) |
| - | * | + | *LED red () 5mm (Roithner LaserTechnik LED750-03AU) |
| - | * | + | *Resistor network 56 Ohms (Distrelec ref number: 71 52 75) |
| + | *Resistor network 82 Ohms (Distrelec ref number: 71 52 81) | ||
| + | *DC Power supply of 5V | ||
| + | |||
| + | <br> | ||
| + | |||
| + | '''Configuration''' | ||
| + | |||
| + | As the forward voltage of the blue LEDs is 3.5V and the nominal current is 20 mA, we mounted each blue LEDs in serial with a resistance of 82 Ohms. | ||
| + | |||
| + | For | ||
<br> | <br> | ||
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<br> | <br> | ||
| - | The initial cell culture is made in dark state. | + | The initial cell culture is made in dark state. Then we illuminate them in the incubator, with constant shaking. To do so, we use small erlenmeiers to have a sufficient amount of culture for the experiments and so that the cells are in a sufficient medium to grow. |
| - | We aslo use the red LEDs | + | We aslo use the red LEDs while loading our samples for further experiments as the LovTAP respond only to a wave lenght. |
</div><div CLASS="epfl09bouchon"></div> | </div><div CLASS="epfl09bouchon"></div> | ||
Revision as of 17:13, 20 October 2009
Material
- LED blue (458nm) 3mm (Distrelec ref number: 63 12 48)
- LED red () 5mm (Roithner LaserTechnik LED750-03AU)
- Resistor network 56 Ohms (Distrelec ref number: 71 52 75)
- Resistor network 82 Ohms (Distrelec ref number: 71 52 81)
- DC Power supply of 5V
Configuration
As the forward voltage of the blue LEDs is 3.5V and the nominal current is 20 mA, we mounted each blue LEDs in serial with a resistance of 82 Ohms.
For
Procedure
The initial cell culture is made in dark state. Then we illuminate them in the incubator, with constant shaking. To do so, we use small erlenmeiers to have a sufficient amount of culture for the experiments and so that the cells are in a sufficient medium to grow. We aslo use the red LEDs while loading our samples for further experiments as the LovTAP respond only to a wave lenght.
