"
Team:Imperial College London/Wetlab/Results/OD calibration
From 2009.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
{{Imperial/09/TemplateTop}} | {{Imperial/09/TemplateTop}} | ||
| + | <br> | ||
<br> | <br> | ||
We have calibrated the absorbance at 600nm (O.D.600) with the colony forming units. This allows us to approximate our absorbance data into a cell count number. We can now normalise fluorescence data to obtain the fluorescence produced per cell.<br> | We have calibrated the absorbance at 600nm (O.D.600) with the colony forming units. This allows us to approximate our absorbance data into a cell count number. We can now normalise fluorescence data to obtain the fluorescence produced per cell.<br> | ||
Latest revision as of 22:47, 20 October 2009
We have calibrated the absorbance at 600nm (O.D.600) with the colony forming units. This allows us to approximate our absorbance data into a cell count number. We can now normalise fluorescence data to obtain the fluorescence produced per cell.
We performed the experiment and further reruns with 2-3 repeats per OD value and for 2 different dilutions.
