Team:Warsaw/Biosafety

Overwiew

As it can be found at our Resources page, we have cloned, or attempted to, some interesting parts from enteropathogenic bacteria, like Salmonella enterolitica ser typhimurium LT2, Yersinia tuberculosis O:8 (8081) and Listeria monocytogenes EGD-E. As pathogenic microorganisms all their derivatives (and of course just themselves) are potentially hazardous.

Biohazard organisms

All steps of isolation of DNA from pathogenic organisms like Yersina tuberculosis O:8 (8081) or Salmonella enterolitica ser. typhimurium LT2 were done under the supervision of people trained and responsible for work with this hazardous material. Team members having contact with Salmonella or Yersinia cultures were special trained and have worked in specially allocated room. Genomic DNA was isolated using standard protocol and then treted like standard genomic DNA sample.

==Potentially hazardous parts

We have managed to prepare listeriolysin from Listeria monocytogenes EGD-E as a biobrick part (. Due to its involvement in pathogenicity of Listeria and as it can be possible that bacteria expressing just listeriolysin may be able to invade eucaryptic cells, special safety measures should be taken in account, especially when working with bacteria transformed with this part. It includes obligatory use of laboratory gloves, and avoidance of any contact with transformed bacteria. All waste contaminated with bacteria expressing listeriolysin should be autoclaved.

and attempted to make internalinA  from  Listeria monocytogenes EGD-E

Environmental safety