Team:Slovenia/Linker-extension standard.html
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- | Figure 1: Schematic presentation of basic elements of two linker-extension standards also named BB-NIC-II and BB-NIC-III vectors | + | Figure 1: Schematic presentation of basic elements of two linker-extension standards also named BB-NIC-II and BB-NIC-III vectors. |
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+ | <center> <img src="https://static.igem.org/mediawiki/2009/8/8c/Linker_ext_Figure_2.gif" align="center" width="600" height="450" border="0" /> | ||
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+ | Figure 2: Schematic presentation of linker extension using both variations of linker-extension standards. A basic BRICK is re-cloned into the linker-extension standard using suitable restriction sites to obtain either Thr-Gly, Ser-Gly or Pro-Gly extensions. | ||
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Revision as of 08:18, 21 October 2009
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New developed linker-extension standard
![]() ![]() Figure 1: Schematic presentation of basic elements of two linker-extension standards also named BB-NIC-II and BB-NIC-III vectors. ![]() Figure 2: Schematic presentation of linker extension using both variations of linker-extension standards. A basic BRICK is re-cloned into the linker-extension standard using suitable restriction sites to obtain either Thr-Gly, Ser-Gly or Pro-Gly extensions. Linker extension
Detailed cloning instructionsDetailed cloning instruction using the linker-extension standard A or the linker-extension standard B, BioBrick-NIC-III, are described in Figure 4 (link) and Figure 5 (link). |