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Notebook/June.html
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(New page: {{SLOtemp| Content= __NOTOC__ ===June 30th, 2009=== Gel electrophoresis of PCR products (see June 29th). BL21DE3 pLysS cells transformed with vectors, containing d53 and Dimtetra, were in...) |
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| + | ===June 29th, 2009=== | ||
| + | We isolated vectors containing d53 and Dimtetra. Gel electrophoresis showed that isolation was successful. | ||
| + | BL21DE3 pLysS cells (for protein production) were transformed with the isolated vectors.<br> | ||
| + | Other group of BL21DE3 pLysS cells was inoculated on LB plates with chloramphenicol. | ||
| + | <br> | ||
| + | <br> | ||
| + | We multiplied ccdB domain and p53 monomer with PCR using ccdB-f, ccdB-r, p53-f and p53-r primers. | ||
| + | <br> | ||
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===June 30th, 2009=== | ===June 30th, 2009=== | ||
| - | Gel electrophoresis of PCR products (see June 29th). | + | Gel electrophoresis of PCR products (see June 29th).<br> |
BL21DE3 pLysS cells transformed with vectors, containing d53 and Dimtetra, were inoculated into 10 mL of LB+antibiotic. | BL21DE3 pLysS cells transformed with vectors, containing d53 and Dimtetra, were inoculated into 10 mL of LB+antibiotic. | ||
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Revision as of 13:34, 21 October 2009
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June 29th, 2009We isolated vectors containing d53 and Dimtetra. Gel electrophoresis showed that isolation was successful.
BL21DE3 pLysS cells (for protein production) were transformed with the isolated vectors. June 30th, 2009Gel electrophoresis of PCR products (see June 29th). |
