Revision as of 14:00, 21 October 2009

Meetings Calendar

Metal Sequester

Introduction

In order to take up and keep the cadmium from the soil efficiently, we needed to think about a way to cross-link the metal ions to intra-cellular proteins which would end up for the most of it in the spore. From our meeting with Prof. Nigel Robinson on the 18th of March 2009, it was evident that the best way of getting cadmium into the spore is to express a metallothionein, which would 'soak up' the cadmium, which in turn would be trapped within the protein in the spore. Using the smtA metallothionein from E.coli and the CotC coat protein as a fusion partner, the metallothionein could in theory be localised to the spore coat as it forms (under control of mother cell specific SigK).

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 ==Introduction==
+In order to take up and keep the cadmium from the soil efficiently, we needed to think about a way to cross-link the metal ions to intra-cellular proteins which would end up for the most of it in the spore.
 From our meeting with Prof. Nigel Robinson on the 18th of March 2009, it was evident that the best way of getting cadmium into the spore is to express a metallothionein, which would 'soak up' the cadmium, which in turn would be trapped within the protein in the spore.
 Using the ''smtA'' metallothionein from ''E.coli'' and the ''CotC'' coat protein as a fusion partner, the metallothionein could in theory be localised to the spore coat as it forms (under control of mother cell specific SigK).
-Craig's 2p:
-It looks as though about 25 - 30% of the cell volume ends up in the forespore (and ultimately the spore).
-I'll assume that our cell was "full" cadmium at the formation of the forespore, because we have knocked out or repressed cadmium efflux.
-So cadmium will not be effluxed from the forespore back to the mother cell or out into the environment.
 ==Novelty in this sub-project==

Team:Newcastle/Metals

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Revision as of 14:00, 21 October 2009

Metal Sequester

Introduction

Novelty in this sub-project

Modelling

BioBrick constructs

Lab Work Strategies

Other Presentations and Diagrams