Virginia Commonwealth/2 July 2009
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- | == | + | ==Thursday 2 July 2009== |
+ | ===Results=== | ||
+ | ''Afton and Maria'' | ||
+ | *All replated (-) controls grew on both KAN plates (lots of little colonies) | ||
+ | **Troubleshooting this problem will be abandoned and new cells will be grown up and tested to ensure the protocols are being done correctly | ||
+ | *The one visible colony on the psB3k3 p1010 backbone with DB 3.1 have grown. It's still just one colony but it is much bigger. | ||
+ | *Overnight culture was successful | ||
+ | ---- | ||
+ | |||
+ | ===Tasks=== | ||
+ | ''Afton and Maria'' | ||
+ | * MiniPrep overnight culture | ||
+ | * Make stocks from overnight culture to freeze | ||
+ | * Begin planning out/documenting Project A in Microsoft Word. | ||
+ | **the goal is to keep a running draft | ||
+ | ---- | ||
+ | |||
+ | ====Wetlab==== | ||
+ | ''Afton and Maria'' | ||
+ | *MiniPrep was done | ||
+ | *Cryogenic storage of DB 3.1 with backbone was frozen in the -80 degree Celsius freezer | ||
+ | [[User:Trentay|Trentay]] 18:48, 2 July 2009 (UTC) |
Revision as of 18:48, 2 July 2009
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Contents |
Thursday 2 July 2009
Results
Afton and Maria
- All replated (-) controls grew on both KAN plates (lots of little colonies)
- Troubleshooting this problem will be abandoned and new cells will be grown up and tested to ensure the protocols are being done correctly
- The one visible colony on the psB3k3 p1010 backbone with DB 3.1 have grown. It's still just one colony but it is much bigger.
- Overnight culture was successful
Tasks
Afton and Maria
- MiniPrep overnight culture
- Make stocks from overnight culture to freeze
- Begin planning out/documenting Project A in Microsoft Word.
- the goal is to keep a running draft
Wetlab
Afton and Maria
- MiniPrep was done
- Cryogenic storage of DB 3.1 with backbone was frozen in the -80 degree Celsius freezer
Trentay 18:48, 2 July 2009 (UTC)