"
Team:KU Seoul/Notebook
From 2009.igem.org
(→Decision making) |
(→Decision making) |
||
| Line 71: | Line 71: | ||
==Decision making== | ==Decision making== | ||
| - | + | '''From Hyukjin Ko, 19th of October''' | |
Our last lab meeting will be held tomorrwo, 8 p.m. | Our last lab meeting will be held tomorrwo, 8 p.m. | ||
| Line 77: | Line 77: | ||
| - | + | '''From Ingeol Choi, 19th of October''' | |
1. Finish off Wiki. | 1. Finish off Wiki. | ||
| Line 86: | Line 86: | ||
| - | + | '''From Ingeol Choi, 16th of October''' | |
[Fwd: Greeting from the HKU-HKBU iGEM team] | [Fwd: Greeting from the HKU-HKBU iGEM team] | ||
| Line 92: | Line 92: | ||
| - | + | '''From Young Byun, 16th of October''' | |
I have ordered our Team T shirt(finally). | I have ordered our Team T shirt(finally). | ||
| Line 796: | Line 796: | ||
IGEM 2007 team has already tried mercury metal sensor, but they failed to make it work. | IGEM 2007 team has already tried mercury metal sensor, but they failed to make it work. | ||
| - | ( | + | (https://2007.igem.org/MIT) |
I think this is due to lack of appropriate biobricks registered. | I think this is due to lack of appropriate biobricks registered. | ||
I am looking through other teams to study. | I am looking through other teams to study. | ||
| Line 1,043: | Line 1,043: | ||
'''From Hanseung Roh, 22nd of July''' | '''From Hanseung Roh, 22nd of July''' | ||
| - | + | https://2007.igem.org/jam07media/Jam07_Glasgow.pdf | |
Check it out. | Check it out. | ||
Latest revision as of 17:15, 21 October 2009
DiscussionFrom instructors
We had a review on our two ideas from Prof.Choi. He told us the ideas are creative and good but not fit for IGEM's concept. We need more feedback and information on our ideas. We need to get more familiar with IGEM's tools. Two additional ideas about Scromboid food poisoning and lactic acid degradation were referred at the end.
Till our next meeting, we should study how systemic biology works and understand related tools. Cumulate ideas. In other words, brainstorm consistently. Hyuk-jin will help with setting up experiments. Lab note opend.
We narrowed our ideas in to two. They are "Metal Detector" and "Bactaeria Compass." Think about what parts would be used and how you would build the circuits. Visit the former iGEM teams and study how they carried on their projects.
Find out the exact mechanism of metal detection. Look up the parts that are available on iGEM home. See how various metals can be detected by using E.coli.
We decided to detect 4 metals; Cd, Hg, Pb, and Ars. They are all critical to the health if ingested. Think about what the outcome would be after detection. Because there are 4 metals, you can bring out 16 different results by combining them. It seems that the most vialbe instrument would be light.
Study what parts on the registry we can use and how you would build a circuit. Increase your knowdlege on circutis by studying former iGEM teams. Remember, imitation is the mother of creation.
Vacation is almost over. The parts that we have looked for are mostly not available in the iGEM 2009 distribution registry, except for lead. We might have to construct the parts ourselves. Once we have our parts and our circuit models, doing experiments is a piece of cake. It's time to fuel up. Look for sponsors.
Think about where you can recieve support. Make a rough plan on where you are going to visit for sponsorship. Divide the team in three groups and give them different work to do.
School started and I know you are all busy. However, we can not postpone our experiments in the name of 'lack of time.' Hyukjin is working hard to help, inspite of his other experiments he needs to work on. Young and Hansung should update our wiki more often. Order our team T-shirts and figure out where we are going to sleep on 29th. Send in the parts.
It's mid term exam period and only two weeks left to Jamboree. Finish our wiki update. Remember, all the judgings are done on the basis of team Wiki. Fill out the judging form as well. BrainstromingIdeas- Biosurfactants - Keratinase - Deodorant - Perfume-producing bacteria - Heat absorber - Bio pencil - Bacteria Array Should we focus on the result, or the procedure? Decision makingFrom Hyukjin Ko, 19th of October Our last lab meeting will be held tomorrwo, 8 p.m. I know it's mid term, but try to come.
1. Finish off Wiki. 2. Judging from. 3. Make poster and prepare for presentation.
[Fwd: Greeting from the HKU-HKBU iGEM team] A letter frin HKU team.
I have ordered our Team T shirt(finally). They are coming next week.
Logo size was changed as requested. Thanks for the help, Hansung. There are still much to do, though. Mid term exam is coming up. Sigh.
I updated our wiki. We need to change our logo size to 965 X 235, Young.
1. Search the induction requirements in papers, and compare them. Don't rely on just one paper. Take into account all possible information. 2. Use the plate reader, which I have advised several times. Try to make a calibration curve as well. 3. Try to grow the cells in liquid broth as well.
The results came out. It is a relief that they came out as expected, but they may have have some controversies we need to talk about. Visit the lab and check our results. We will also use the plate reader.
[Fwd: [iGEM Interest] iGEM Social Event] Check it out.
How was Choosuk everyone? Our meeting tomorrow will be on 5 p.m. as usual. Let's have a nice dinner together. It's getting cold.
1. Register the parts before deadline. 2. Use the plate reader to read the results. 3. If the results are good, organize them into a paper.
I have confirmed the sequences and the cloning worked out well. It's time to check if the promoters actually work. We will make the heavy metal stock first.
So far, so good. Update Wiki!!! Try to get more support as well. I will talk to the dean myself.
Plane tickest are confirmed. I will attend the orientation, Cheolwon. Let's pray that the results will come out as expected.
Horray!!! We were judged suitable for CCP. That means we receive $3000 and additional $1000 if the results are good! Thank God, we can pay off some of our plane ticket costs.
I have class on that time.
We got a new chance for sponsorship! The information was brought forth by Jihee. By registering our iGEM team as a formal school club, we can attend the club contest. If we win, we can receive $3000. Let's go for it.
The CCP's result will come out on 30th of September. The team T-shirt design was done by Cheolwoo's friend, and we think it's gorgeous. We will order them by the end of the month. Thanks to Hyukjin, our experiment is flowing on course. Merry Choosuk, everyone.
Our meeting today will be at the same spot, same time. We will discuss about Team T shirts and plane tickets. See you all.
The prices are really good.
The plane tickets seem nice. Go make the reservations.
The PCR results came out well, except for the vectors. I will rerun the vector PCRs in different conditions. The PCR results will be attatched.
http://air.interpark.com/lts/jsp/discountCity.jsp?SaleType=M&areaCode=U1&CityCode=LAX&aNationCode=US&mbn=tourair&mln=air_country04&tdOpen=Y#f_air It's a discount ticket from Incheon -> Narita -> New York -> Boston for only $1000!!! It might be annoying to wait for planes during intervals, but I think the cheap price beats it. We will depart on 29th of October and arrive on 3rd of November. There are not that many seats left, so I think we should make reservation till this weekend.
I am terribly sorry for the delay. I am not trying to make excuses, but I had severe fever and almost forgot about the abstract. I sent it today. I just wish it's not too late.
[Fwd: Missed iGEM deadline: Project abstract and title] What's going on guys?
There's a Korean Synthetic Biology conference this week. If you have anything to present, send them to me. Those who are interested are welcome to join me.
I will look over your abstract, Cheolwon. I think it's great. From Hyukjin Ko, 19th of September I thank those who came to the meeting today. We finished plasmid extraction. The attatched file is its result. We will run PCR when the ordered primers arrive on Tuesday. I will post the ppt file for today's meeting tomorrow.
I made our team abstract. My poor English fails me. Our team project is related to the detection system for heavy metals which are harmful for environment, such as arsenic, mercury, and cadmium. The purpose of this project is to detect the concentration of heavy metals in the waste water with mixed color system. We use several flurosence proteins GFP RFP and amd gene as detection markers. Each heavy metal promoter produce matched flurosence protein. For examples, High concentration of Arsenic could induce the green flurosence with GFP proteins. Such system works very well as accurate as ppb (parts per billion concentration) Finely, the mixed heavy metals produce gradient and mixed colors according to light mixed rules. This system is more effective than previous circuits because of integrated analysis.
Make sure Jamboree registration is done. Update wiki, Young and Hansung. Design Team T-shirts.
We transformed our parts to E. coli DH5a and ccdB survival strain in the morning. We wll work on plasmid mini prep. and agarose gel electrophoresis after supper.
The meeting will include: 1. primer design & cloning method 2. discussion for refined design It's not compulsory, so those who are not interested don't have to come.
I am sure most of the members know this, but reviewing is always a good idea :)
Register the Jamboree members, Hansung.
The ppt file is from the presentation I made on Tuesday. It includes all the explanation. As I have mentioned on Tuesday, we will be using degradation tag to remove already expressed proteins.
Members expected to come at the scheduled hour should be here on time. I am planning to give detailed explanation on the primers on Saturday.
The hotels have been reserved. Hyatt Regency 10/30(Fri)~11/2(Mon) (3 nights) Check out the flight plans also. There is no direct flight from Incheon to Boston. Progress is slow, but I think we are on track. Burn your fuels.
Graduates stay in the lab most of the day, so don't worry about our schedules. Drop by any time. :) I will try to post our actual plans tonight.
I will make an excel file that shows everyone's available time. Send me your free time schedule that you are willing to spend for our experiments.
I will visit Professor Choi on Monday and discuss final decisions. We need to amplify the required genes, and grow cells to extract genomic DNA. Additional plates are also needed. I will explain more about the details on Tuesday, 5 p.m. It is mandatory that all members attend.
Now that's what I call useful information. Way to go, Young.
I just read a paper on YodA protein!!! It's a cadmium specific protein. It is normally kept at a low level, but when Cadmium is induced to the cell, the concentration goes up 50 times. The beautiful aspect is that it is very specific for cadmium and no other metals.
I found this paper using LazZ and lux to detect mercury by attatching them to mercury promoter PmerTPAD and Cu promotoer PcoE. Cu showed gradual response to concentration, but mercury only showed threshold response. I think Lux is a good tool for detection we could try out on our experiment.
The big companies are not cooperative either. Sigh.
I think cadmium sensing protein CadC could be used. I think they are not included in the iGEM distribution, but it works in E.coli. Part:BBa_K112406
Our final brochure.
The papers Keena presented were completely irrelevant. We need to use microbes that detect heavy metals using a specific system. These papers seem to explain human related genes and their relationship with heavy metals.
Ars, the information Young sent me was quite useful.
Professor and other students are not working on the project because they got plenty of time left. It's your iGEM. Work on it.
See you all.
I am not sure if these are useful, but I think it contains some information. Give me feedback. http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2535653
1. What about circuits for other metals? 2. How will you use AMD as a reporter? 3. Any simulations?
The meeting held on Friday was great. Almost all members attended the meeting: Hansung, Hyukjin, Jihee, Jihae, Young, Simin, Cheolwoo, Yeonjin, and me. Sadly, Yeonjin, Keena and Yoonjoo is quitting iGEM project due to personal reasons. Young, Jihee, Jihae, Hansung, and I am going to attend the Jamboree.
The problem at hand is what parts we are going to use. We will be also looking for sponsors next week. Have a great week all !
I tried to post it on the weekends, but something came up. It's a simple draft of the circuit. Explanations are included in the files attatched. Good night everyone.
[Fwd: Team KU_Seoul: Check In 2] It's a letter from HQ.
Have the bill sent to my lab. Find out who is joining the Jamboree. I need to make reservations for the hotel.
We can try to receive supports based on that.
As I have announced before, we will meet on this Friday and have dinner together. I wish all the members come. 6 p.m. West Department 1st floor.
I have tried various corporates and associations, but they are not that willing to support us. I do understand that it might be waste of money for them, but it is really disappointing. The attatched file includes addresses to few places that gave relatively positive response. I am planning to visit them next week. Anyone who wants to go, text me.
https://2009.igem.org/Team:KU_Seoul Color selections are fine, but try to make them brighter. They are kind of dark.
I have changed the design and colors of our main page. See if it's okay and give me comments so I can fix it. I will apply it to every other sections.
The vacation is almost over, and 2nd semester is coming up. I am planning to have a meeting next Friday, 4th of September. We will be using the lab after class, so approximate time for our experiments would be around 5 p.m. Have a nice weekend.
Nice job, Young. I will help you.
I have updated the Notebook section of our wiki. The discussion we had during July has been updated. I have a hard time figuring out how to link pictures with other sites. Someone help me out.
Nice work, Young. Try to update wiki as well.
I have sent the Preliminary team track selection. I considered it urgent. We need to visit iGEM home more often.
1. Most metal detectors can be used in E.coli. I think we can make them with PCR. 2. Look for parts for other metals. If they are not registered, let us do the job. The brochure is fine, but try to make it look more professional. Remind yourself that it's not to introduce me, but to introduce Team_KU. The Samsung sponsoring did not work out well. They say their budget plan is full.
I don't remember sending preliminary team track selection, which probably means nobody did.
Sorry for the late letter. Mercury detecting part BBa_I728456 and other parts are all not included in the 2009 distribution plate, which means we would not be able to use them. You can check available parts here. http://partsregistry.org/Help:IGEM_09_DNA_distribution I am currently on vacation, but I will try to build a circuit using AMD. Have a nice vacation!!!
Here's our brochure. Comments are welcome.
What do you mean by Preliminary team track selection? Have we sent it? Are we on the right track?
Visit me on Monday. [Fwd: [iGEM Interest] The MathWorks offer deadline and Preliminary track selections] Check the letter.
Yes, I will have the brochure done by 23rd. We will discuss about the participants of CCP on Friday. I also need your approval of our team. May I visit your office on Monday?
Seems like a good chance. Try to make a brochure as well. I am planning to visit Samsung next Tuesday, and I need it.
CCP stands for Creative Challenger Program. It supports teams like us, but we need to register and compete to be selected. Let's give it a try.
The metal promoters registered do not seem to work. http://partsregistry.org/Promoters/Catalog/Metal_sensitive
This is an Arsenite part I found on the web. There is no Arsenite part registered on iGEM currently. Let us register it. http://biocyc.org/ECOLI/NEW-IMAGE?type=OPERON&object=TU00239
From Ingeol Choi, 17th of August If we are thinking three inputs, we can use the tri-stable switch. http://openwetware.org/wiki/IGEM:Brown/2007/Tri-Stable_Switch Professor Kwangil Ko is working on something similar. You might want to visit his lab. Update your wiki!
I think we have found all the sensors for lead, arsenic, and mercury. We should now focus on how to express them. For example, if we mix up GFP, YFP, RFP, would the results come out as a mixed fluorescence? We can also use pigments, but we must look for them. The amd gene professor recommended seems like a good candidate. However, if we are to express them differently, we need two more pigments. Let's look for them.
1. You can sense and bind mercury with MerR ( However, it might bind other metals as well.) 2. It would be great if we bind all four different metals simultaneously( which should be hard). 3. Talk with Hyukjin for experiment planning. 4. Synthetic Biology team is going on a trip for three days. We won't be able to make it on Friday meeting.
IGEM 2007 team has already tried mercury metal sensor, but they failed to make it work. (https://2007.igem.org/MIT) I think this is due to lack of appropriate biobricks registered. I am looking through other teams to study. Hope we can all meet on Friday. See you all.
1. Signal in: Mercury detection: MerR - mercury binding protein regulator 2. Signal out: amd gene cloned by Hyukjin (Able to detect with Tyrenol) References http://mic.sgmjournals.org/cgi/content/abstract/152/3/709 http://www.ncbi.nlm.nih.gov/pubmed/10503547 http://www.sciencemag.org/cgi/content/abstract/301/5638/1383 (copper?)
I designed our mascot as I mentioned. I will put it up on wiki. See how it looks.
https://2009.igem.org/Judging has been posted. See what is needed. Confirm who's going to the Jamboree.
I finished our brocheure. Let's make a list of places to visit for our fund raising.
Participants : Youngseol Byun, Hansung Roh, Hyukjin Ko, Cheolwoo Lee Discussion 1 : We need to study the mechanisms for our parts. Understand how they work. Discussion 2 : We are going to have our detector show the difference in concentration. Check out the former iGEM team that worked on Vanillin. Conclusion : Study circuits and how to build them.
Jamboree registration starts at last. Get excited!!!
How is creating our mascot a bacteria holding a metal detctor? I will work on it.
Thanks for the feedbacks. Can you guys send me your thoughts about iGEM to put them in our brocheure. Slogans for the brocheure are also welcome.
We need a decent title for the brocheure. How's 'We design life!!! - Metal detecting cell' ? We need something strong!
I agree with Young. I made a brocheure for our fund raising. I need feedback.
I think the logo should contain something to do with our project. Nice work, though.
I made our logo for iGEM. They come in two different colors. See and how you like it. Give me suggestions. From Ingeol Choi, 3rd of August Don't worry. Team Gronigen's project and ours is quite different. Update our project discription for wiki.
Thanks everyone for your participation inspite of vacation. I refined my ideas and made some fixes with the diagram I sent before. See what changed.
Team Gronigen seems to do something similar with us. https://2009.igem.org/Team:Groningen Check it out.
Among the teams of iGEM 2007, there's a device that compares A and B's concentration. I think we can use this to figure out which metal is contained more. I have looked up the parts for Lead, Cadmium, Mercury as Cheolwon had suggested. As for lead, it is confirmed that it works well but for cadmium and mercury we have to try them ourselves. I added an excel file that shows the parts I looked up.
I looked up some parts in the registry with the keyword Metal. It seems like there are not many that we can use. How is everyone else going?
Here is a schematic diagram of biosensor. Your opinions are very welcome.
Send me your photos and a short description of yourself by this weekend. Mottos and your message to iGEM are very welcome. Have a terrific weekend !!!
Okay, guys. Here is a sum up of our votes, though I don't understand why you sent your votes through private mails. Our focus would be "Bacteria Array" and "Bacteria Compass."
Mascot Design : Yeonjin Kong, Yoonji Kim, Kina Jeon Parts Study : Young Byun, Hansung Roh Circuit Design : Hyukjin Ko, Cheolwon Choi Brocheure : Cheolwon CHoi Those who couldn't attend our meeting, help out any groups you would like lend a hand on. As for the parts study, make a list with Excel or whatever you would like to use, and explain how they work. Biobrick numbers registered on iGEM is also crucial. I am planning of a party? to encourage our team. Post me your convenient time. P.S. I almost forgot. Use Google group unless it's something VERY private !!! Don't send your mails directly to me. :)
I have my votes for "Bacteria Array." I also read an interesting article on Bacillus pasteruii which concretes sand into cement using calcium carbonate. It was first invented to solidify bedrocks along beaches but is now planned to build sand walls around deserts to prevent its growth. These are the links: http://cedb.asce.org/cgi/WWWdisplay.cgi?0611182 http://www.popularmechanics.co.za/content/news/singlepage.asp?key=229 http://www.news.ucdavis.edu/search/news_detail.lasso?id=8040 http://www.environmentalgraffiti.com/featured/bacteria-sahara-desertification/11121
I made a list of our ideas accumulated till now. You have two votes. Choose two subjects that you think are feasible, interesting, and suitable for synthetic biology. Have your votes sent by Wednesday.
Remember Kina's idea about geosmin? This is an article about geosmin determined as a criteria for choosing drinkable water. http://www.iipc.co.kr/notice/notice1_view.asp?id=notice&bbsid=notice1&board_id=notice&ref=111&step=1&RefLevel=0¤t_page=
As I was looking through some papers, I read about mussels. A Korean science team devloped bio-glue from mussels that live attatched to rocks in the ocean. The problem is that the amount produced is scarce, which we can solve by using microbiology. Bioglue seems attractive.
To team members. I have read all the mails and they are great. Try to develop more ideas on the environment part. - Make a list of ideas and categorize them. - Do not think about if they would work or not, yet. That's not brainstorming. - If you want to be more specific, think about what parts or circuits you could use for the idea. Have you all studied what synthetic biology is? Check out http://partsregistry.org/Catalog. It might help you out. The file included contains my suggestions about your ideas. Have a nice day everyone.
Hey guys. I wish there were more exchange of ideas by Google. Don't keep your ideas to yourself. Share them with others and discuss freely. Don't think that your ideas are not worth it.
There are people still confused about how to use Google groups. It is extremely SIMPLE. Send your mail to synbiogroup@googlegroups.com and all the team members recieve your mail. Yeap, that's all you need to do.
Scrombroid food poisoning is the 2nd prevalent food posioning among fish and its main cause is histamine. Histidine which exists in all fish turns into histamine by histidine decarboxylase at temperature above 16 degrees celcius. Histmine is also noteworthy as a substrate for the expression of alergic reactions. The basic mechanism is using "Band defect network" that was introduced in 'Towards Logical Designs in Biology.' GFP expression is repressed at low levels of histamine but strongly expressed at above threshold level. By this way, we can tell if the fish is edible or not. We are using a well known circuit here, so it might be a nice try. I thought about the ideas our team produced. I think Bacteria Array is fascinating. But as for the aroma producing bacteria, can the small amount of fragrance produced overwhelm the stink of rotting food?
If there is a way, I want to develop a way to increase the survival rates of diesel producing bacterias. This idea is also good for raising funds if we succeed! Can we also make bio gasolines?
There is a molecule called Geosmin produced by microbes in water. It giveas the water a dirt flavor, an important factor for drinking water. We could build a bacteira that can sense geosmin and trap it, like the one Chiva university did on iGEM 2007.
As I was searching the text books, I thought of making a "Bacteria Array," similar to DNA array. It can be built to sense specific substrates in a compound.
Gees. It poured cats and dogs during the weekends. Your ideas are due Monday. I appreciate your full participation.
From Simin Kim, 15th of July Hey people. How's an aroma producing bacteria? If we can make a bacteria that can produce fragrance by using wasted food, we might not need to buy Febreeze anymore! :)
Hi everyone. The two subjects that we thought were good turned out to be not that suitable for iGEM. We need MORE ideas!!! We also need a clearer concept on synthetic biology. These are homeworks due to our next meeting, 17th of July. 1) https://2009.igem.org/Instructional_Videos Watch the videos. 2) Study the videos on iGEM 2009 home and tell us what you learned. 3) Divide our team into groups for each subject. 4) We need to learn more about Wiki. Wiki seems to take great part in judging. Although we are not that good in English, try to post your ideas. Try to visit our team Wiki everyday. 5) Please!!! Post me if you can't come.
This mail has a linked file that contains the lists of our team member's e-mails and contacts.
Hi everyone. We narrowed our ideas to two subjects, which are "Morse Code" and "Bio Sticker." If you would like to share any other ideas, you are more then welcome. Let's discuss about specific models for these two subjects at the upcoming meeting. Our next meeting is on 10th of July, 3 p.m. I recommend you think about at least one of the two subjects and how they should be developed. Tell me your schedules via mail so that I can fix our meeting time to the most suitable for all. I would like to share my experience at the iGEM workshop at Dongkyung about Wiki and other stuffs that you might be interested, so bring your notebooks. As I said before, prepare a specific model for the meeting. Try to document your ideas so they could be shared with others. A specific model should include these: 1. How will the chosen subject be used? 2. What are its limits? 3. What materials would be needed? 4. What former knowledge would be required to process the subject? 5. Why did you choose the subject? 6. What contributions can you make? 7. Etc. See you next Friday. Korean stuffs
|
