Team:PKU Beijing/Notebook/AND Gate 1/Input/TetR 2

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[[Team:PKU_Beijing/Notebook|Notebook]] > [[Team:PKU_Beijing/Notebook/AND_Gate_1|AND Gate 1]] > [[Team:PKU_Beijing/Notebook/AND_Gate_1/Input|Input]] > [[Team:PKU_Beijing/Notebook/AND_Gate_1/Input/TetR_2|aTc Sensor]]
==='''2009.8.16'''===
==='''2009.8.16'''===

Revision as of 18:44, 21 October 2009
 
Notebook > AND Gate 1 > Input > aTc Sensor

2009.8.16

Test if the tetR promoter system works well in the low-copy backbone.
Get tetR+promoter+GRP from Wu Shuke.

16:40
Digest the promoter and reporter system.

Total50μL
Plasmids5μL
EcoR11μL
Pst11μL
Buffer2μL
ddH2O11μL

20:00
Electrophoresis to recycle the inserts.
The order of the samples: marker, digestion products, plasmids control.
Results:
PKU 20090816 Shan Shen 1.JPG
Only the first one is correctly digested, recycle them.

2009.8.17

10:00
Link the inserts with vectors with has Kanamycin resistance.

17:50
Transformation.

19:30
Start to incubate.

2009.8.18

10:00
There are many colonies on the plate.
PCR colonies to test if they are correct.

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