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Team:Cambridge/Project/Amplification
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| - | = | + | = Sensitivity Tuners = |
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| + | ==Previous Work== | ||
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| + | Part of the iGEM mission is to build on previous projects. The Cambridge 2007 iGEM team developed a PoPS amplifier system using phage activators and promoters. The system works by using a PoPS input to make an activator protein, as shown in the diagram from their wiki below, which then binds to a promoter and generates a PoPS output. | ||
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| + | [[Image:amplifier07.jpg]] | ||
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| + | In order to quantify the ratio between PoPS in and PoPS out, the team built the following construction on the high copy plasmid pSB1A2, with mRFP and GFP as PoPS reporter. They genenerated 15 total combinations of different activators and promoters. | ||
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| + | [[Image:construction07.jpg]] | ||
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| + | They successfully quantified the PoPS amplification factors for each activator/promoter combination after arabinose induction. | ||
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| + | ==Kit of Parts== | ||
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| + | The Cambridge 2009 team analyzed the properties of these constructs, paying particular attention to how adding phage activator and promoter combinations downstream of pBad altereted the behaviour of pBad. In particular, it caught our attention that the phage activator and promoter combinations | ||
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Revision as of 19:59, 21 October 2009
Categories :
Project :
-
Overview
Sensitivity Tuner
--- Characterisation
--- Modelling
Colour Generators
--- Carotenoids (Orange/Red)
--- Melanin (Brown)
--- Violacein (Purple/Green)
The Future
Safety
Notebook :
Team Logistics :
Sensitivity Tuners
Previous Work
Part of the iGEM mission is to build on previous projects. The Cambridge 2007 iGEM team developed a PoPS amplifier system using phage activators and promoters. The system works by using a PoPS input to make an activator protein, as shown in the diagram from their wiki below, which then binds to a promoter and generates a PoPS output.
In order to quantify the ratio between PoPS in and PoPS out, the team built the following construction on the high copy plasmid pSB1A2, with mRFP and GFP as PoPS reporter. They genenerated 15 total combinations of different activators and promoters.
They successfully quantified the PoPS amplification factors for each activator/promoter combination after arabinose induction.
Kit of Parts
The Cambridge 2009 team analyzed the properties of these constructs, paying particular attention to how adding phage activator and promoter combinations downstream of pBad altereted the behaviour of pBad. In particular, it caught our attention that the phage activator and promoter combinations
