Team:Newcastle/Metalsensing

From 2009.igem.org

(Difference between revisions)
(→Lab Work done)
(→Lab Work done)
Line 37: Line 37:
| '''[https://2009.igem.org/Team:Newcastle/Labwork/18_August_2009#Metal_Sensor_Team 18th August 2009]'''
| '''[https://2009.igem.org/Team:Newcastle/Labwork/18_August_2009#Metal_Sensor_Team 18th August 2009]'''
| Transformed ''DH5-alpha E. coli'' cells with ''BBa_J33206'' from the Spring Distribution
| Transformed ''DH5-alpha E. coli'' cells with ''BBa_J33206'' from the Spring Distribution
 +
|-
 +
|'''[https://2009.igem.org/Team:Newcastle/Labwork/19_August_2009#Metal_Sensor_Team 19th August 2009]'''
 +
| Inoculated 3 tubes of LB with 3 colonies of potential transformant ''E.coli'' cells
|-
|-
|}
|}

Revision as of 20:44, 21 October 2009


Metal Sensing

Introduction

If our project is to process cadmium and not other metals, we need to genetically engineer Bacillus subtilis to carry out a set of cellular processes based on the action of metal sensors. These metal sensors will detect cadmium through a system known as AND Gating.

There are two metal sensing repressors, which are known to respond to cadmium: arsR and czrA. By placing binding sites to these two metal sensing repressors next to each other in a promoter region, the gene regulated by that promoter will be synthesized only when a combination of metals that bind to both sensors are present; this is a combinatorial approach for gene expression regulation.

Modelling

BioBrick constructs

Lab Work Strategies

Other Presentations and Diagrams

Newcastle Metalsensor2100.gif

Lab Work done

Summary of Lab Sessions for Cadmium Sensing
Date
Description
18th August 2009 Transformed DH5-alpha E. coli cells with BBa_J33206 from the Spring Distribution
19th August 2009 Inoculated 3 tubes of LB with 3 colonies of potential transformant E.coli cells



News

Events

Social Net

  • Newcastle iGEM Twitter
  • [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
  • [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]
Retrieved from "http://2009.igem.org/Team:Newcastle/Metalsensing"