Team:Newcastle/Metalsensing
From 2009.igem.org
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|'''[https://2009.igem.org/Team:Newcastle/Labwork/27_August_2009#Metal_Sensor_Team 27th August 2009]''' | |'''[https://2009.igem.org/Team:Newcastle/Labwork/27_August_2009#Metal_Sensor_Team 27th August 2009]''' | ||
- | |Digested ''pSB1A2'' (containing ''BBa_J33206'' BioBrick) | + | |Digested ''pSB1A2'' (containing ''BBa_J33206'' BioBrick), ran DNA though gel and excised band. |
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+ | |'''[https://2009.igem.org/Team:Newcastle/Labwork/28_August_2009#Metal_Sensor_Team] 28th August 2009''' | ||
+ | |Cleaned gel band using Gel extraction kit | ||
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Revision as of 21:13, 21 October 2009
Metal Sensing
Introduction
If our project is to process cadmium and not other metals, we need to genetically engineer Bacillus subtilis to carry out a set of cellular processes based on the action of metal sensors. These metal sensors will detect cadmium through a system known as AND Gating.
There are two metal sensing repressors, which are known to respond to cadmium: arsR and czrA. By placing binding sites to these two metal sensing repressors next to each other in a promoter region, the gene regulated by that promoter will be synthesized only when a combination of metals that bind to both sensors are present; this is a combinatorial approach for gene expression regulation.
Modelling
BioBrick constructs
Lab Work Strategies
Other Presentations and Diagrams
Lab Work done
Summary of Lab Sessions for Cadmium Sensing | |
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18th August 2009 | Transformed DH5-alpha E. coli cells with BBa_J33206 from the Spring Distribution |
19th August 2009 | Inoculated 3 tubes of LB with 3 colonies of potential transformant E.coli cells |
20th August 2009 | Conduct mini-preps on the three overnight-grown cultures of potential BBa_J33206 transformants and digested them with restriction enzymes. |
21st August 2009 | Analysed digested BBa_J33206 mini-prep DNA by DNA gel electrophoresis and prepared midi-preps also. |
25th August 2009 | Concentrated (and ethanol precipitated) the BBa_J33206 Midi-prep sample. |
26th August 2009 | Digested BBa_J33206 midi-prep DNA with EcoRI and PstI and analysed through DNA gel electrophoresis - digest reaction not successful (a second attempt at digests needed) |
27th August 2009 | Digested pSB1A2 (containing BBa_J33206 BioBrick), ran DNA though gel and excised band. |
[1] 28th August 2009 | Cleaned gel band using Gel extraction kit |
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]