Functional biomaterials Idea Approach.html
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Revision as of 22:23, 21 October 2009
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Functional biomaterials
The idea and approachBioactive materials and their design technology enable an extensive range of applications for the medicine and related fields. Numerous biopolymers have been developed as scaffolds supporting growth of cells and tissues. Rationally designed polypeptides offer a promising way for constructing biomaterials. For instance, self-assembling and self-complementary amphiphilic peptides have already been investigated; most extensively among those were beta-sheet and fiber forming peptides (Stupp et al., 2005; Holmes et al., 2000). What could be an advantageous environment for the mammalian cell growth? This question led us to design elastin-based self-assembling polypeptides, which could form new polypeptide material, which mimics extracellular matrix, which is the natural environment of different eucaryotic cells and provides different cell-growth supporting and protecting functions. Namely, the significant stability of elastin and elastin-related polymers and their tendency for self-aggregation make them ideal compounds for the development of synthetic nanomaterials. The precursor of stable, cross-linked elastin is tropoelastin (Figure 1) which is composed of: • hydrophobic domains which contain repetitive sequences, which provide elastomeric properties and can coacervate through temperature driven phenomenon, • hydrophilic domains which contain residues important for cross-linking into stable polymer elastin (Bellingham et al., 2003).
Figure 1: Elastin peptides are aligned during coacervation of hydrophobic domains (square planar structures with spherical structures representing hydrophobic side chains) and can be therefore covalently cross-linked through lysine residues on hydrophilic domains (cylinders) (Keeley et al., 2002). Elastin-like materials containing hydrophobic repetitive sequences have already been prepared (Lee et al., 2001), but to employ them as resilient biomaterials, the polypeptide chains have to be covalently crosslinked. We came to the idea that elastin-like segments (consisted of repetitive hydrophobic domains of elastin) could be cross-linked by strong noncovalent interactions by introduction of coiled-coil forming segments. In addition to coiled-coil segments we can also add other biologically active segments (Figure 2) such as functional domains for inhibition of bacterial growth or mammalian growth factors. Since the functional parts would be covalently incorporated into the scaffold, there would be no need to add them to cells in soluble form, which would also increase their local concentration. Particularly appealing idea seems to be to add functional segments, which have a heterodimeric coiled-coil appendix, which allows them to attach from the solution to the polypeptide matrix through coiled-coil interactions. This would allow addition of a cocktail of different functional elements that could be tailored individually to specific cell or tissue type and desired biological activity.
Figure 2: Schematic presentation of the polypeptide biomaterial composed of elastin-like segments and coiled-coil segments. Coiled-coil segments oligomerize and connect individual polypeptide chains of the biomaterial polypeptide scaffold. Functional polypeptide domains can be either incorporated into the polypeptide chain as part of the fusion protein or can be linked to the scaffold by interactions between coiled-coil segments of the scaffold and coiled-coil segment fused to the functional polypeptide.
Figure 3: Schematic presentation of the regulated assembly of two polypeptides material components. One soluble polypeptide containing a coiled-coil segment capable of heterooligomerization remains soluble. Assembly can be the initiated only after the addition of the second polypeptide component.
Figure 4: Schematic presentation of the disassembly of the polypeptide biomaterial after the addition of the peptide comprising coiled-coil segment (broken line ellipse), which is also present in the polypeptide material. The added coiled-coil segment competes with coiled-coil pairing of the polypeptide material due to its ability to bind to one of the coiled-coil segments of the polypeptide material. This approach can also be used to release the compounds immobilized to the polypeptide matrix through coiled-coil segment. |