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Team:Brown/Notebook Protocols/primerresusp
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(New page: {{Brown}} Primer resuspension ---- Goal: 5pM/ul. This is a 1xstock solution. Protocol: 1) Identify the nM amount on the tube and resuspend the primers in 10x (in ul) water, ...) |
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Revision as of 23:00, 21 October 2009
Primer resuspension
Goal: 5pM/ul. This is a 1x stock solution.
Protocol: 1) Identify the nM amount on the tube and resuspend the primers in 10x (in ul) water, e.g. 23.5 nM would be resuspended in 235 uL water. This is a 100 pM (or 0.1 nM) and is the 20x that you protect! 2) Dilute a portion of this 20x into a fresh tube, appropriately labeled, to make a 1X stock solution, or 5pm/uL. This is your working solution. Use 2uL per reaction. 3) Store at -20oC.
