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Team:BIOTEC Dresden/Results SilverNano
From 2009.igem.org
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=====Spectrophotometry===== | =====Spectrophotometry===== | ||
| - | Fig.1 shows the observed absorption spectrum for silver nanoparticles reported by Naik in doi:10.1038/nmat758. Figure 2 shows the spectrum observed from the incubated sample containing silver binding peptide, silver nitrate and sodium ascorbate. | + | Fig.1 shows the observed absorption spectrum for silver nanoparticles reported by Naik in doi:10.1038/nmat758. Figure 2 shows the spectrum observed from the incubated sample containing silver binding peptide, silver nitrate and sodium ascorbate. That the production of silver nanoparticles is explicitly due to the presence of the peptide AG4 is confirmed by the absence of expected the absorbance peaks (400-500nm) in a negative control containing only buffers, silver nitrate, and sodium ascorbate. |
A good agreement is observed, the deviation can be explained as a result of a different size distribution in our sample. | A good agreement is observed, the deviation can be explained as a result of a different size distribution in our sample. | ||
Revision as of 03:49, 22 October 2009
Silver Nano Particles - Results
Electron Microscopy
Electron micrographs of an incubated sample of silver binding peptide, with Sodium Ascorbate, Silver Nitrate, and water. Crystalline structures indicating the presence metallic particles are clearly visible.
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Spectrophotometry
Fig.1 shows the observed absorption spectrum for silver nanoparticles reported by Naik in doi:10.1038/nmat758. Figure 2 shows the spectrum observed from the incubated sample containing silver binding peptide, silver nitrate and sodium ascorbate. That the production of silver nanoparticles is explicitly due to the presence of the peptide AG4 is confirmed by the absence of expected the absorbance peaks (400-500nm) in a negative control containing only buffers, silver nitrate, and sodium ascorbate.
A good agreement is observed, the deviation can be explained as a result of a different size distribution in our sample.
The protocol to create silver particles works and can be combined with the gene expression in vesicles to influence the size distribution.
