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Team:Warsaw/Calendar-Main/9 July 2009
From 2009.igem.org
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| - | <center><h3>Miecznikowa team: adjusting part BBa_J5528 | + | <center><h3>Miecznikowa team: adjusting part BBa_J5528</h3></center> |
<h4>Franek</h4> | <h4>Franek</h4> | ||
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<p>Methods:</p> | <p>Methods:</p> | ||
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| - | <li>Plates with BBa_I0500 were empty, therefore once again transformation of chemocompetent cells was performed, but this time | + | <li>Plates with BBa_I0500 were empty, therefore once again transformation of chemocompetent cells was performed, but this time 8µl of BBa_I0500 DNA solution was used</li> |
<li>Plating bacterias with BBa_I0500 on LB medium supplemented with kanamycin</li> | <li>Plating bacterias with BBa_I0500 on LB medium supplemented with kanamycin</li> | ||
| - | <li>Resuspension of DNA from plate 1, 2C (BBa_B0024) with | + | <li>Resuspension of DNA from plate 1, 2C (BBa_B0024) with 15µl of H2O</li> |
| - | <li>Transformation of chemocompetent cells with | + | <li>Transformation of chemocompetent cells with 4µl of BBa_B0024 DNA solution</li> |
<li>Plating bacterias with BBa_B0024 on LB medium supplemented with ampicillin</li> | <li>Plating bacterias with BBa_B0024 on LB medium supplemented with ampicillin</li> | ||
</ul> | </ul> | ||
Revision as of 22:29, 11 July 2009
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Isolation of BioBricks from 2008 and 2009 Kit Plates
Monika
Selected BioBricks:
Miecznikowa team: adjusting part BBa_J5528
Franek
Task:
- Alkaline lysis of the plasmid containing BBa_I0500
- Transform competent cells with BBa_B0024
- Second attempt to transform competent cells with BBa_I0500
Methods:
- Plates with BBa_I0500 were empty, therefore once again transformation of chemocompetent cells was performed, but this time 8µl of BBa_I0500 DNA solution was used
- Plating bacterias with BBa_I0500 on LB medium supplemented with kanamycin
- Resuspension of DNA from plate 1, 2C (BBa_B0024) with 15µl of H2O
- Transformation of chemocompetent cells with 4µl of BBa_B0024 DNA solution
- Plating bacterias with BBa_B0024 on LB medium supplemented with ampicillin
Results:
- Will be determined tomorrow
Jarek
Task:
- Isolation of plasmid containing parts from liquid cultures
- Digestion of acquired samples with restriction endonucleases
- Electrophoretic separation of digested samples
- Isolation of samples from agarose gel
Methods:
- Plasmid DNA was isolated with A&A "Plasmid Mini" kit, the DNA concentration was measured with nanodrop
- For digestion 1 ul of PstI/SpeI (B0032) or PstI/XbaI enzymes and 2 ul of 1xTango buffer were used. Digestion was held for 3 hours.
- After digestion samples were separated due to elecrophoresis in 0,8 agarose gel in TBE buffer
- Samples were isolated from gel with A&A "Gel-out" kit
Results:
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