Selected BioBricks:

Franek

Task:

• Alkaline lysis of the plasmid containing BBa_I0500
• Transform competent cells with BBa_B0024
• Second attempt to transform competent cells with BBa_I0500

Methods:

• Plates with BBa_I0500 were empty, therefore once again transformation of chemocompetent cells was performed, but this time 8µl of BBa_I0500 DNA solution was used
• Plating bacterias with BBa_I0500 on LB medium supplemented with kanamycin
• Resuspension of DNA from plate 1, 2C (BBa_B0024) with 15µl of H2O
• Transformation of chemocompetent cells with 4µl of BBa_B0024 DNA solution
• Plating bacterias with BBa_B0024 on LB medium supplemented with ampicillin

Results:

• Will be determined tomorrow

Jarek

Task:

• Isolation of plasmid containing parts from liquid cultures
• Digestion of acquired samples with restriction endonucleases
• Electrophoretic separation of digested samples
• Isolation of samples from agarose gel

Methods:

• Plasmid DNA was isolated with A&A "Plasmid Mini" kit, the DNA concentration was measured with nanodrop
• For digestion 1 ul of PstI/SpeI (B0032) or PstI/XbaI enzymes and 2 ul of 1xTango buffer were used. Digestion was held for 3 hours.
• After digestion samples were separated due to elecrophoresis in 0,8 agarose gel in TBE buffer
• Samples were isolated from gel with A&A "Gel-out" kit

Results: