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Team:TUDelft/13 July 2009
From 2009.igem.org
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Tim Weenink (Talk | contribs) (→Weenink) |
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Also did the first transformations to see how it goes: | Also did the first transformations to see how it goes: | ||
| - | BBa_K145280 GFP-LVA + TetR generators | + | BBa_K145280 GFP-LVA + TetR generators <br> |
BBa_K145279 GFP + TetR generators | BBa_K145279 GFP + TetR generators | ||
| Line 15: | Line 15: | ||
after addition of 1 µl rehydrated biobrick DNA, the tubes were incubated for approx 15 (±5) min. on ice. Then one tube of each transformation was heatshocked for 1 min at 42 degrees. | after addition of 1 µl rehydrated biobrick DNA, the tubes were incubated for approx 15 (±5) min. on ice. Then one tube of each transformation was heatshocked for 1 min at 42 degrees. | ||
| - | All four transformations were plated out and put at 37 degrees (at 19:00) | + | All four transformations were plated out and put at 37 degrees (at 19:00) <br> |
| - | BBa_K145280 no heatshock approx 80 microliter on agar+amp plate | + | BBa_K145280 no heatshock approx 80 microliter on agar+amp plate<br> |
| - | BBa_K145279 no heatshock 50 microliter on agar+amp plate | + | BBa_K145279 no heatshock 50 microliter on agar+amp plate<br> |
| - | BBa_K145280 heatshocked 50 microliter on agar+amp plate | + | BBa_K145280 heatshocked 50 microliter on agar+amp plate<br> |
| - | BBa_K145279 heatshocked 50 microliter on agar+amp plate | + | BBa_K145279 heatshocked 50 microliter on agar+amp plate<br> |
{{Template:TUDelftiGEM2009_end}} | {{Template:TUDelftiGEM2009_end}} | ||
Revision as of 21:40, 13 July 2009
13th July
Weenink
prepared 10 agar+amp plates
Also did the first transformations to see how it goes:
BBa_K145280 GFP-LVA + TetR generators
BBa_K145279 GFP + TetR generators
4 vials of DH5alpha comptetent cells were used (2 for each biobrick)
after addition of 1 µl rehydrated biobrick DNA, the tubes were incubated for approx 15 (±5) min. on ice. Then one tube of each transformation was heatshocked for 1 min at 42 degrees.
All four transformations were plated out and put at 37 degrees (at 19:00)
BBa_K145280 no heatshock approx 80 microliter on agar+amp plate
BBa_K145279 no heatshock 50 microliter on agar+amp plate
BBa_K145280 heatshocked 50 microliter on agar+amp plate
BBa_K145279 heatshocked 50 microliter on agar+amp plate
