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Team:Groningen/Notebook/14 July 2009
From 2009.igem.org
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(→GVP Cluster) |
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''GVP eluted in MQ'' | ''GVP eluted in MQ'' | ||
| - | * | + | * 329.7 ng/μL |
| - | * | + | * 1.84 (260/280) |
| - | * | + | * 2.13 (260/230) |
| + | |||
| + | ''[http://partsregistry.org/wiki/index.php?title=Part:BBa_J23109 BBa_J23109] eluted in MQ'' | ||
| + | * 174.9 ng/μL | ||
| + | * 1.88 (260/280) | ||
| + | * 1.92 (260/230) | ||
| + | |||
| + | ''[http://partsregistry.org/wiki/index.php?title=Part:BBa_J23100 BBa_J23100] eluted in MQ'' | ||
| + | * 155.3 ng/μL | ||
| + | * 1.85 (260/280) | ||
| + | * 1.79 (260/230) | ||
| + | |||
| + | ''[http://partsregistry.org/wiki/index.php?title=Part:BBa_J23106 BBa_J23106] eluted in MQ'' | ||
| + | * 219.4 ng/μL | ||
| + | * 1.86 (260/280) | ||
| + | * 1.93 (260/230) | ||
===Transporters=== | ===Transporters=== | ||
Revision as of 09:37, 14 July 2009
[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
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Wet
GVP Cluster
Plasmid Purification
Plasmid isolation was performed on the cultures of GVP and Terminator containing cells with the "Sygma-Aldrich™ GenElute™ Plasmid Miniprep Kit".
- From each tube 5mL of culture was collected in a 2mL cup, and the cells were pelleted by centrifugation for 1 min. at max. speed and the supernatant discarded.
- Cells were resuspended in 200μL Resuspension Solution by up and down pipetting.
- To the mixture 200μL Lysis Solution was added, mixed by inverting the cup, and stored at room temperature for 5 minutes.
- 350μL of Neutralisation Solution was added and the tubes inverted.
- Cell debri was pelleted by centrifugation at full speed for 10 min.
- Column was prepared by adding 500μL Column Preparation Solution and centrifuging for 1 min. full speed
- The lysate was transfered to the column and centrifuged for 1 min. full speed
- Column was washed with both washing solutions
- Plasmids were eluted with 30μL MQ and stored in the fridge
Concentration of Plasmids
The concentration of isolated plasmid was determined with the use of a nano-drop.
GVP eluted in MQ
- 329.7 ng/μL
- 1.84 (260/280)
- 2.13 (260/230)
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J23109 BBa_J23109] eluted in MQ
- 174.9 ng/μL
- 1.88 (260/280)
- 1.92 (260/230)
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J23100 BBa_J23100] eluted in MQ
- 155.3 ng/μL
- 1.85 (260/280)
- 1.79 (260/230)
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J23106 BBa_J23106] eluted in MQ
- 219.4 ng/μL
- 1.86 (260/280)
- 1.93 (260/230)
Transporters
Dry
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