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-= Week from July 6th, to July 12nd, 2009 =
+= Week from July 13rd, to July 19th, 2009 =
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-<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week1Jul">
+<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week2Jul">
   <img src="https://2009.igem.org/Special:FilePath?file=Left_arrow.png"/>
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-<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week1Jul">Previous Week</a>
+<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week2Jul">Previous Week</a>
 </td>
 <td align="right" width="50%">
-<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week3Jul">Next Week</a>
+<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week4Jul">Next Week</a>
-<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week3Jul">
+<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week4Jul">
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-== <html><font class="dayw_style">July, 6th</font></html> ==
+== <html><font class="dayw_style">July, 13rd</font></html> ==
-*We planned to dedicate this week to the assembly of an IPTG/lactose->PoPS device in order to have a lactose sensor for our project, but also a useful inducible system for general purpose applications.
-*We already had R0011 and BOL1 purified plasmids stored at -20°C. Digestion for:
-{|cellpadding="20"
-|R0011(E-X)
-|BOL1(E-S)
-|}
-*Gel run/cut and band purification.
-*The purified DNA gave a good result at Nanodrop, but we decided to perform the entire work on BOL1 again because we were not sure that we had extracted a pure band.
-*We stored R0011(E-X) DNA at -20°C.
-*We infected 5 ml of LB + Amp with 8 ul of BOL1 glycerol stock to grow two identical overnight cultures (37°C, 220 rpm).
-''Preparation of experiment with Tecan F200''
-*We picked a colony of B0030 plate (stored at +4°C) and infected 5 ml of LB + Amp.
-*We also infected 5 ml of LB + Amp with 10 ul of A1 and J23100 glycerol stocks.
-*We incubated the inocula overnight at 37°C, 220 rpm.
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-== <html><font class="dayw_style">July, 7th</font></html> ==
-*Miniprep for BOL1 (X2).
-*Digestion for BOL1(E-S)(X2).
-*Gel run/cut/purification: none of the two BOL1 samples gave a good results in DNA quantification...we decided to cut the miniprepped plasmids again, but this time through an overnight digestion (both plasmids E-S).
-*In case of failure, we also infected 5 ml of LB + Amp with 8 ul of BOL1 glycerol stock to grow an overnight culture at 37°C, 220 rpm.
-*We prepared 0.5 l of LB + Amp.
-*We received sequencing results for:
-**T9002 - long part, but SEQUENCE CONFIRMED! while MIT results were "INCONSISTENT".
-**A10 - results showed a good chromatogram, but K117000 had not been correctly ligated. We will repeat this ligation one of the next weeks.
-''Preparation of experiment with Tecan F200''
-*We diluted 1:100 the overnight cultures of A1, B0030 and J23100.
-*We incubated the diluted cultures for 5 hours (37°C, 220 rpm).
-''Experiment with Tecan F200''
-* <html><u>Description</u></html>
-* <html><u>Purpose:</u></html>
-* <html><u>Materials & Methods</u></html>
-* <html><u>Protocol</u></html>
-* <html><u>Results</u></html>
-''Preparation of tomorrow's experiment with Tecan F200''
-*We picked a colony from B0030 native plate (stored ad +4°C) and infected 5 ml of LB + Amp. We incubated the inoculum at 37°C, 220 rpm overnight.
+== <html><font class="dayw_style">July, 14th</font></html> ==
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-== <html><font class="dayw_style">July, 8th</font></html> ==
-*Gel run/cut/band purification for BOL1(E-S) overnight digestion. Results: gel showed that the enzymes had cut very well and this time also the purification gave a good quantification at Nanodrop.
+== <html><font class="dayw_style">July, 15th</font></html> ==
-*We took R0011(E-X) stored ad -20°C and we were ready to ligate;)
-*Ligation:
-**A11: BOL1(E-S) + R0011(E-X) in pSB1A2
-*We incubated the ligation overnight at 16°C.
-''Preparation of experiment with Tecan F200''
-*We diluted 1:100 the overnight culture of B0030.
-''Experiment with Tecan F200 (continues the next day)''
-* <html><u>Description</u></html>
-* <html><u>Purpose:</u></html>
-* <html><u>Materials & Methods</u></html>
-* <html><u>Protocol</u></html>
-* <html><u>Results</u></html>
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@@ Line 138: / Line 43: @@
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-== <html><font class="dayw_style">July, 9th</font></html> ==
-*We resuspended F2620 BioBrick from iGEM 2009 plates.
+== <html><font class="dayw_style">July, 16th</font></html> ==
-*We transformed about 40 pg of the overnight ligation and 1 ul of the resuspended F2620 BioBrick. We plated transformed bacteria and incubated the two plates overnight at 37°C.
-''End of experiment with Tecan F200 (last measurement)''
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-== <html><font class="dayw_style">July, 10th</font></html> ==
-*A11 and F2620 overnight plates showed colonies!
-*Colony PCR for 6 colonies of A11 plate: the picked colonies were inoculated in 1 ml of LB + Amp and incubated at 37°C, 220 rpm waiting for PCR results.
-*We also picked a colony from F2620 to infect 1 ml of LB + Amp. We incubated this inoculum with the others.
-<font class='didascalia'>
-{|align="center"
-|[[Image:pv_colonypcr_A11.jpg|thumb|300px|left|Colony PCR for A11 plate: all the colonies showed the correct plasmid. We kept the 1st, 3rd and 6th colonies.]]
-|}
-</font>
-*Gel results: all the picked colonies showed the expected amplicon of a correctly ligated plasmid! We decided to prepare a glycerol stock for:
-**A11-1
-**A11-3
-**A11-6
-*Next week, only A11-1 will be used, but the other colonies will be used in case of failure.
-*We also prepared a glycerol stock for F2620 culture.
+== <html><font class="dayw_style">July, 17th</font></html> ==
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Team:UNIPV-Pavia/Notebook/Week3Jul

From 2009.igem.org

Revision as of 09:03, 16 July 2009

Week from July 13rd, to July 19th, 2009

July, 13rd

July, 14th

July, 15th

July, 16th

July, 17th