Virginia Commonwealth/13 July 2009
From 2009.igem.org
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* Back bone pSB3K3 is a bad part. Cultivate backbone pSB4C5 from glycerol stock. | * Back bone pSB3K3 is a bad part. Cultivate backbone pSB4C5 from glycerol stock. | ||
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+ | ''Craig and Clay'' | ||
+ | * It was decided to continue work done by the 2008 Eidenburg team by engineering a metabolic pathway for limonene synthesis. Three parts are currently available in the distribution plates (BBa I742111, BBa K118024, and BBa K118025). BBa I742111 contains a ribosomal binding site attached to the limonene synthase gene (LIMS1) from citrus lemon. BBa K118024 contains the former as well as ribosomal binding sites and a dxs gene and appY gene. BBa K118025 contains the ribosomal binding sites and the three aforementioned genes as well as a promoter. We will begin by electrotransforming these parts into the NEB 10 beta strain of E. coli once the electrotransformation cuvettes arrive. | ||
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====Wetlab==== | ====Wetlab==== |
Revision as of 16:20, 17 July 2009
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Contents |
Monday 13 July 2009
Results
Maria and Afton
- Digestion was successful on parts J23106 and J06702
Trentay 18:30, 13 July 2009 (UTC)
Tasks
Maria and Afton
- Make daily planner for experimentation
- Perform Electrophoresis test on Digested parts J23106 and J06702
- Ligate parts: J23106 (promoter), J06702 (RET), pSB4C5 (backbone)
- Transform ligated DNA into e. coli NEB 10 beta
Trentay 18:34, 13 July 2009 (UTC)
Kevin and Adam
- Back bone pSB3K3 is a bad part. Cultivate backbone pSB4C5 from glycerol stock.
- 2
Craig and Clay
- It was decided to continue work done by the 2008 Eidenburg team by engineering a metabolic pathway for limonene synthesis. Three parts are currently available in the distribution plates (BBa I742111, BBa K118024, and BBa K118025). BBa I742111 contains a ribosomal binding site attached to the limonene synthase gene (LIMS1) from citrus lemon. BBa K118024 contains the former as well as ribosomal binding sites and a dxs gene and appY gene. BBa K118025 contains the ribosomal binding sites and the three aforementioned genes as well as a promoter. We will begin by electrotransforming these parts into the NEB 10 beta strain of E. coli once the electrotransformation cuvettes arrive.
Wetlab
Maria and Afton
- Gel Electrophoresis
- Digestion of J23100
- Transformation of ligated parts: J23106 (promoter), J06702 (RET), pSB4C5 (backbone)
- Also Ligated: J23100 (promoter), J06702 (RET), pSB4C5 (backbone)
- Plated:
- (+) Control: NEB 10 beta on LB
- (+) Control: NEB 10 beta on LB shocked
- (-) Control: NEB 10 beta on Cm
- NEB 10 beta with BioBrick part (J23106, J06702, pSB4C5)
Trentay 20:26, 13 July 2009 (UTC)
Kevin and Adam
- grew plasmids pSB4C5, E0240, and J06702 from glycerol stocks. Will be mini prepped in the morning.
Bussingkm 22:59, 13 July 2009 (UTC)