Team:TUDelft/22 July 2009
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===Sriram=== | ===Sriram=== | ||
- | Observed growth in all the 5 culture tubes | + | Observed growth in all the 5 culture tubes [BBa_E0040 - GFP, BBa_C0040 - TetR, BBa_C0051 - cl, BBa_I12006 - λp, BBa_E1010 - mRFP1] and at 11 AM the DNA extraction was started and done with miniprep plasmid DNA extraction kit. The DNA concentrations were noted for all the amplified biobrick parts using Nanodrop. [The values must be given below...It is in lab notes ... will be updated tomorrow...] |
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+ | Agarose gel electrophoresis was run for all the 12 parts amplified with 2µl loading buffer for 20 minutes (17:30 to 17:50)and the gel is stored in fridge to continue with electrophoresis tomorrow. | ||
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===Daniel=== | ===Daniel=== | ||
Revision as of 18:45, 22 July 2009
22 July 2009
Sriram
Observed growth in all the 5 culture tubes [BBa_E0040 - GFP, BBa_C0040 - TetR, BBa_C0051 - cl, BBa_I12006 - λp, BBa_E1010 - mRFP1] and at 11 AM the DNA extraction was started and done with miniprep plasmid DNA extraction kit. The DNA concentrations were noted for all the amplified biobrick parts using Nanodrop. [The values must be given below...It is in lab notes ... will be updated tomorrow...]
Agarose gel electrophoresis was run for all the 12 parts amplified with 2µl loading buffer for 20 minutes (17:30 to 17:50)and the gel is stored in fridge to continue with electrophoresis tomorrow.
Daniel
Calin