Team:TUDelft/22 July 2009
From 2009.igem.org
(→22 July 2009) |
(→22 July 2009) |
||
Line 6: | Line 6: | ||
Observed growth in all the 5 culture tubes [BBa_E0040 - GFP, BBa_C0040 - TetR, BBa_C0051 - cl, BBa_I12006 - λp, BBa_E1010 - mRFP1] and at 11 AM the DNA extraction was started and done with miniprep plasmid DNA extraction kit. The DNA concentrations were noted for all the amplified biobrick parts using Nanodrop. [The values must be given below...It is in lab notes ... will be updated tomorrow...] | Observed growth in all the 5 culture tubes [BBa_E0040 - GFP, BBa_C0040 - TetR, BBa_C0051 - cl, BBa_I12006 - λp, BBa_E1010 - mRFP1] and at 11 AM the DNA extraction was started and done with miniprep plasmid DNA extraction kit. The DNA concentrations were noted for all the amplified biobrick parts using Nanodrop. [The values must be given below...It is in lab notes ... will be updated tomorrow...] | ||
- | Agarose gel electrophoresis was run for all the 12 | + | Agarose gel electrophoresis was run for all the 12 amplified biobrick parts with 2µl loading buffer for 20 minutes (17:30 to 17:50)and the gel is stored in fridge to continue with electrophoresis tomorrow. |
===Daniel=== | ===Daniel=== | ||
- | Prepared the glycerol stocks for the remaining culture of the 9 biobricks [BBa_E0040 - GFP, BBa_C0040 - TetR, BBa_C0051 - cl, BBa_I12006 - λp, BBa_E1010 - mRFP1 of Delay module and ] for future use. The protocol is as follows: | + | Prepared the glycerol stocks for the remaining culture of the 9 biobricks [BBa_E0040 - GFP, BBa_C0040 - TetR, BBa_C0051 - cl, BBa_I12006 - λp, BBa_E1010 - mRFP1 of Delay module and BBa_I714031 - OriT-R, BBa_J23100 - strong promoter, BBa_E0840 - GFP generator and BBa_I13522 - pTet GFP of Conjugation module] for future use. The protocol is as follows: |
1. Centrifuge 1ml of culture in eppendorf at 8000rpm for 3 minutes at 15°C.<br> | 1. Centrifuge 1ml of culture in eppendorf at 8000rpm for 3 minutes at 15°C.<br> | ||
Line 22: | Line 22: | ||
===Calin=== | ===Calin=== | ||
- | Observed growth in all the | + | Observed growth in all the 4 culture tubes [BBa_I714031 - OriT-R, BBa_J23100 - strong promoter, BBa_E0840 - GFP generator and BBa_I13522 - pTet GFP] and at 11 AM the DNA extraction was started and done with miniprep plasmid DNA extraction kit. The DNA concentrations were noted for all the amplified biobrick parts using Nanodrop. [The values must be given below...It is in lab notes ... will be updated tomorrow...] |
- | Agarose gel electrophoresis was run for all the 4 | + | Agarose gel electrophoresis was run for all the 4 amplified biobrick parts, with 2µl loading buffer for 20 minutes (17:30 to 17:50)and the gel is stored in fridge to continue with electrophoresis tomorrow. |
{{Template:TUDelftiGEM2009_end}} | {{Template:TUDelftiGEM2009_end}} |
Revision as of 18:55, 22 July 2009
22 July 2009
Sriram
Observed growth in all the 5 culture tubes [BBa_E0040 - GFP, BBa_C0040 - TetR, BBa_C0051 - cl, BBa_I12006 - λp, BBa_E1010 - mRFP1] and at 11 AM the DNA extraction was started and done with miniprep plasmid DNA extraction kit. The DNA concentrations were noted for all the amplified biobrick parts using Nanodrop. [The values must be given below...It is in lab notes ... will be updated tomorrow...]
Agarose gel electrophoresis was run for all the 12 amplified biobrick parts with 2µl loading buffer for 20 minutes (17:30 to 17:50)and the gel is stored in fridge to continue with electrophoresis tomorrow.
Daniel
Prepared the glycerol stocks for the remaining culture of the 9 biobricks [BBa_E0040 - GFP, BBa_C0040 - TetR, BBa_C0051 - cl, BBa_I12006 - λp, BBa_E1010 - mRFP1 of Delay module and BBa_I714031 - OriT-R, BBa_J23100 - strong promoter, BBa_E0840 - GFP generator and BBa_I13522 - pTet GFP of Conjugation module] for future use. The protocol is as follows:
1. Centrifuge 1ml of culture in eppendorf at 8000rpm for 3 minutes at 15°C.
2. Decant the supernatant.
3. Add 1 ml of culture and centrifuge at 8000rpm for 3 minutes at 15°C.
4. Decant the supernatant.
5. Add 1 ml of culture and centrifuge at 8000rpm for 3 minutes at 15°C.
6. Decant the supernatant.
7. Add 1 ml of culture and centrifuge at 8000rpm for 3 minutes at 15°C.
8. Decant 0.5ml of the supernatant.
9. Resuspend the dense pellet throughly, add 0.5 ml of 10% glycerol and store in -80°C freezer.
Calin
Observed growth in all the 4 culture tubes [BBa_I714031 - OriT-R, BBa_J23100 - strong promoter, BBa_E0840 - GFP generator and BBa_I13522 - pTet GFP] and at 11 AM the DNA extraction was started and done with miniprep plasmid DNA extraction kit. The DNA concentrations were noted for all the amplified biobrick parts using Nanodrop. [The values must be given below...It is in lab notes ... will be updated tomorrow...] Agarose gel electrophoresis was run for all the 4 amplified biobrick parts, with 2µl loading buffer for 20 minutes (17:30 to 17:50)and the gel is stored in fridge to continue with electrophoresis tomorrow.