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Team:TUDelft/23 July 2009
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looked at gel | looked at gel | ||
| - | Did !A construction with double the amount of backbone (and rest from fridge). Did it with both DH5alpha and homemade top10 chemically competent cells. | + | Did !A construction with double the amount of backbone (and rest from fridge). Did it with both DH5alpha and homemade top10 chemically competent cells, to compare transformation efficiencies. |
PCR'd 8 colonies from my transformation plates with VF2 and I-SceI Reverse primers to see if there was incorporation of the I-SceI restriction site | PCR'd 8 colonies from my transformation plates with VF2 and I-SceI Reverse primers to see if there was incorporation of the I-SceI restriction site | ||
Revision as of 19:08, 23 July 2009
Tim Weenink
Details of the following to be provided tomorrow:
grew up *S in LC
looked at gel
Did !A construction with double the amount of backbone (and rest from fridge). Did it with both DH5alpha and homemade top10 chemically competent cells, to compare transformation efficiencies.
PCR'd 8 colonies from my transformation plates with VF2 and I-SceI Reverse primers to see if there was incorporation of the I-SceI restriction site
isolated plasmid from *S
