"
Team:Warsaw/Calendar-Main/24 July 2009
From 2009.igem.org
(Difference between revisions)
| Line 16: | Line 16: | ||
<li>Despite the fact that all transferred colonies turned more or less blue the purification was carried out using the Plasmid Mini kit (A&A Biotechnology) according to the manufacturers protocol.</li> | <li>Despite the fact that all transferred colonies turned more or less blue the purification was carried out using the Plasmid Mini kit (A&A Biotechnology) according to the manufacturers protocol.</li> | ||
<li>The digest mix was prepared as fallows: 10μl of purified plasmid, 2μl Tango buffer (Fermentas), 1μl SpeI enzyme, 1μl XbaI enzyme, the solution was topped up with H2O to the final volume of 20μl.</li> | <li>The digest mix was prepared as fallows: 10μl of purified plasmid, 2μl Tango buffer (Fermentas), 1μl SpeI enzyme, 1μl XbaI enzyme, the solution was topped up with H2O to the final volume of 20μl.</li> | ||
| - | <li>The digest was incubated for 3h | + | <li>The digest was incubated for 3h at 37°C. |
<li>The results were visualised with gel electrophoresis on 1% agarose gel. | <li>The results were visualised with gel electrophoresis on 1% agarose gel. | ||
</ul> | </ul> | ||
| Line 24: | Line 24: | ||
<li>Gel Electrophoresis:</li> | <li>Gel Electrophoresis:</li> | ||
</ul> | </ul> | ||
| + | <p>Sukcesy | ||
| + | <p> | ||
| + | <p>Nigdy mi nie odmówiły | ||
| + | <p>Te, które mi się śniły. | ||
| + | <p>(Jan Sztaudynger) | ||
<br /> | <br /> | ||
</html> | </html> | ||
Revision as of 12:43, 24 July 2009
 |  |
 |
  |
 |
|
Cloning of the mgtc promoter into the pKSII+ plasmid
Kamil
Tasks:
- Plasmid purification
- Control digest
Methods:
- Despite the fact that all transferred colonies turned more or less blue the purification was carried out using the Plasmid Mini kit (A&A Biotechnology) according to the manufacturers protocol.
- The digest mix was prepared as fallows: 10μl of purified plasmid, 2μl Tango buffer (Fermentas), 1μl SpeI enzyme, 1μl XbaI enzyme, the solution was topped up with H2O to the final volume of 20μl.
- The digest was incubated for 3h at 37°C.
- The results were visualised with gel electrophoresis on 1% agarose gel.
Results:
- Gel Electrophoresis:
Sukcesy
Nigdy mi nie odmówiły
Te, które mi się śniły.
(Jan Sztaudynger)
|
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
