"
Team:TUDelft/24 July 2009
From 2009.igem.org
(→Tim Vos) |
(→24 July 2009) |
||
| Line 6: | Line 6: | ||
The cultures were grown in all the culture tubes [R0040, J23008, J23031, B0015, K081013, S03335, S03473, I714031, E0840, J23100]incubated yesterday except RBS[B0034] and pLacI[R0010], which may be because the colony we picked for those biobricks were not perfect. The plasmid DNA was extracted from all the grown cultures and stored in -20°C freezer. | The cultures were grown in all the culture tubes [R0040, J23008, J23031, B0015, K081013, S03335, S03473, I714031, E0840, J23100]incubated yesterday except RBS[B0034] and pLacI[R0010], which may be because the colony we picked for those biobricks were not perfect. The plasmid DNA was extracted from all the grown cultures and stored in -20°C freezer. | ||
| + | The biobricks received from MIT for Delay [S03335, S03473] which were streaked yesterday have the colonies for future. | ||
===Tim Vos=== | ===Tim Vos=== | ||
| - | Glycerol stocks were prepared for the cultures grown in all the culture tubes [R0040, J23008, J23031, B0015, K081013, S03335, S03473, I714031, E0840, J23100]incubated yesterday and stored in -80°C freezer. | + | Glycerol stocks were prepared for the remaining cultures grown in all the culture tubes [R0040, J23008, J23031, B0015, K081013, S03335, S03473, I714031, E0840, J23100]that were incubated yesterday and stored in -80°C freezer. |
| + | |||
| + | ===Daniel=== | ||
| + | Since the top10 competent cells newly prepared by us using the Openwetware protocol, didn't transform well for Tim Weenink we are in a bit crisis for competent cells. Anyway to make sure whether the cells work or not I did transformation of Biobricks RBS[B0034] and pLacI[R0010] in the newly created top10 competent cells and kept in drawer to grow in the weekend. | ||
{{Template:TUDelftiGEM2009_end}} | {{Template:TUDelftiGEM2009_end}} | ||
Revision as of 14:25, 24 July 2009
24 July 2009
Sriram
The cultures were grown in all the culture tubes [R0040, J23008, J23031, B0015, K081013, S03335, S03473, I714031, E0840, J23100]incubated yesterday except RBS[B0034] and pLacI[R0010], which may be because the colony we picked for those biobricks were not perfect. The plasmid DNA was extracted from all the grown cultures and stored in -20°C freezer.
The biobricks received from MIT for Delay [S03335, S03473] which were streaked yesterday have the colonies for future.
Tim Vos
Glycerol stocks were prepared for the remaining cultures grown in all the culture tubes [R0040, J23008, J23031, B0015, K081013, S03335, S03473, I714031, E0840, J23100]that were incubated yesterday and stored in -80°C freezer.
Daniel
Since the top10 competent cells newly prepared by us using the Openwetware protocol, didn't transform well for Tim Weenink we are in a bit crisis for competent cells. Anyway to make sure whether the cells work or not I did transformation of Biobricks RBS[B0034] and pLacI[R0010] in the newly created top10 competent cells and kept in drawer to grow in the weekend.
