Team:Newcastle/Labwork/27 July 2009

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Revision as of 14:50, 27 July 2009


Today we prepared the gel for last week's rescued plasmids whcih contain RFP and GFP biobricks. WE prepared the gel with 0.8% agorose. The final solution was 500ml using 4 gr of agorose in total.

We checked the agar plates containing colonies of plasmids with five different biobricks. Plate 1: Bba_C0056 Plate 2: Bba_B1002 Plate 3: Bba_ Plate 4: Bba_C0076 Plate 5: Bba_R0077

Expcept from plate 3 we had colonies formed in all plates.

we prepared 12 tubes for 4 different biobricks. Colonies were picked and mixed in a tube containing 5ml of LB. Finally we left them at shaking incubator overnight. We labelled the tubes as below: 1A: 1B: 1C: We then prepared the plates for the plasmids.





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