Team:Newcastle/Labwork/27 July 2009
From 2009.igem.org
(Difference between revisions)
Goksel (Talk | contribs)
(New page: {{:Team:Newcastle/CSS}} {{:Team:Newcastle/Header}} {{:Team:Newcastle/Left}} Today we prepared the gel for last week's rescued plasmids whcih contain RFP and GFP biobricks. WE prepared the...)
Newer edit →
(New page: {{:Team:Newcastle/CSS}} {{:Team:Newcastle/Header}} {{:Team:Newcastle/Left}} Today we prepared the gel for last week's rescued plasmids whcih contain RFP and GFP biobricks. WE prepared the...)
Newer edit →
Revision as of 14:50, 27 July 2009
Today we prepared the gel for last week's rescued plasmids whcih contain RFP and GFP biobricks. WE prepared the gel with 0.8% agorose. The final solution was 500ml using 4 gr of agorose in total.
We checked the agar plates containing colonies of plasmids with five different biobricks. Plate 1: Bba_C0056 Plate 2: Bba_B1002 Plate 3: Bba_ Plate 4: Bba_C0076 Plate 5: Bba_R0077
Expcept from plate 3 we had colonies formed in all plates.
we prepared 12 tubes for 4 different biobricks. Colonies were picked and mixed in a tube containing 5ml of LB. Finally we left them at shaking incubator overnight. We labelled the tubes as below: 1A: 1B: 1C: We then prepared the plates for the plasmids.
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]