Team:Newcastle/Labwork/27 July 2009

From 2009.igem.org

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Today we prepared the gel for last week's rescued plasmids whcih contain RFP and GFP biobricks. WE prepared the gel with 0.8% agorose. The final solution was 500ml using 4 gr of agorose in total.
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=Lab Session 27/07/2009=
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 +
Today we prepared the gel for last week's rescued plasmids which contain RFP and GFP biobricks. We prepared the gel with 0.8% agorose. The final solution was 500ml using 4 gr of agorose in total.
 +
 
 +
We checked the agar plates containing colonies of plasmids with five different biobricks from last week.
 +
The list of plates and the biobricks are below:
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We checked the agar plates containing colonies of plasmids with five different biobricks.
 
{| class="wikitable" border="1"
{| class="wikitable" border="1"
|-
|-
| Plate
| Plate
| BioBrick
| BioBrick
 +
| Number
|-
|-
| Plate 1
| Plate 1
| Bba_C0056
| Bba_C0056
 +
| 1
|-
|-
| Plate 2
| Plate 2
| Bba_B1002
| Bba_B1002
 +
| 2
|-
|-
| Plate 3
| Plate 3
| Bba_??
| Bba_??
 +
| 3
|-
|-
| Plate 4
| Plate 4
| Bba_C0076
| Bba_C0076
 +
| 4
|-
|-
| Plate 5
| Plate 5
| Bba_R0077
| Bba_R0077
 +
| 5
|-
|-
|}
|}
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Expcept from plate 3 we had colonies formed in all plates.
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Expcept from plate 3, we had colonies formed in all plates.
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we prepared 12 tubes for 4 different biobricks. Colonies were picked and mixed in a tube containing 5ml of LB. Finally we left them at shaking incubator overnight.
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Colonies from the plates were picked and mixed in a tube containing 5ml of LB. We prepared 12 tubes for 4 different biobricks. For each biobrick we labelled the tubes as A, B, and C. Finally we left them at shaking incubator overnight.
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We labelled the tubes as below:
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1A:
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-
1B:
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-
1C:
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-
We then prepared the plates for the plasmids.
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 +
==Preparation of the plates==
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We prepared the agar solutions are poured into the plates which are then placed into the fridge.
 +
The list of plates and their content are as below
 +
{| class="wikitable" border="1"
 +
|-
 +
| Content
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| Number of plates
 +
|-
 +
| LB
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| 4
 +
|-
 +
| LB + Amp
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| 6
 +
|-
 +
| LB + Amp + Kan
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| 5
 +
|-
 +
|}
{{:Team:Newcastle/Footer}}
{{:Team:Newcastle/Footer}}
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{{:Team:Newcastle/Right}}

Revision as of 15:02, 27 July 2009

Lab Session 27/07/2009

Today we prepared the gel for last week's rescued plasmids which contain RFP and GFP biobricks. We prepared the gel with 0.8% agorose. The final solution was 500ml using 4 gr of agorose in total.

We checked the agar plates containing colonies of plasmids with five different biobricks from last week. The list of plates and the biobricks are below:

Plate BioBrick Number
Plate 1 Bba_C0056 1
Plate 2 Bba_B1002 2
Plate 3 Bba_?? 3
Plate 4 Bba_C0076 4
Plate 5 Bba_R0077 5

Expcept from plate 3, we had colonies formed in all plates.

Colonies from the plates were picked and mixed in a tube containing 5ml of LB. We prepared 12 tubes for 4 different biobricks. For each biobrick we labelled the tubes as A, B, and C. Finally we left them at shaking incubator overnight.

Preparation of the plates

We prepared the agar solutions are poured into the plates which are then placed into the fridge. The list of plates and their content are as below

Content Number of plates
LB 4
LB + Amp 6
LB + Amp + Kan 5



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