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EPF-Lausanne/16 July 2009
From 2009.igem.org
(New page: ===Wet Lab=== Our plates and tubes from yesterday transformation are the same for the negative control and the LovTAP-Term. So either the ligation or the transformation didn't work. Actual...) |
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Our plates and tubes from yesterday transformation are the same for the negative control and the LovTAP-Term. So either the ligation or the transformation didn't work. Actually we think that it is the transformation, because we put the wrong antibiotics : Chl instead of Amp. As we don't have any ligation products any more, we did the digestion, gel extraction and ligation again. | Our plates and tubes from yesterday transformation are the same for the negative control and the LovTAP-Term. So either the ligation or the transformation didn't work. Actually we think that it is the transformation, because we put the wrong antibiotics : Chl instead of Amp. As we don't have any ligation products any more, we did the digestion, gel extraction and ligation again. | ||
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: Heidi, Tu, Nath, Caro, Nicolas | : Heidi, Tu, Nath, Caro, Nicolas | ||
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Revision as of 07:01, 28 July 2009
Contents |
Wet Lab
Our plates and tubes from yesterday transformation are the same for the negative control and the LovTAP-Term. So either the ligation or the transformation didn't work. Actually we think that it is the transformation, because we put the wrong antibiotics : Chl instead of Amp. As we don't have any ligation products any more, we did the digestion, gel extraction and ligation again.
We also did a PCR of LacI-RBS product of the second transformation (of 15.07.09), as the concentration for the transformation of 14.07.09 was too low. We then ran the gel.
Gel extraction of Term.
Preparation of LB plates : AMP plates and Chl plates.
Then ligation of LovTAP and Term.
Finally transformation of the ligation product (with Amp and not Chl antibiotics this time !!) and Term.
People in the lab
- Heidi, Tu, Nath, Caro, Nicolas
