From 2009.igem.org
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- | ===27.07.09===
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- | LOVTAP gene have been amplified again and runned on a gel to be sure to have the correct insert.
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- | [http://partsregistry.org/Part:BBa_B0015 BBa_B0015 (terminator)] was digested, runned on a gel (which displayed the correct band) and extracted with the gel extraction kit, this step didn't worked well.
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- | LOVTAP gene have been than digested, purified and runned on a gel.
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- | We already had a digested [http://partsregistry.org/Part:BBa_B0015 BBa_B0015 (terminator)].
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- | Finally we ligated overnight at 4°C the digested LOVTAP in both [http://partsregistry.org/Part:BBa_B0015 terminators], the one digested-extracted today and the one already digested-extracted.
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| ===28.07.09=== | | ===28.07.09=== |
Revision as of 08:02, 28 July 2009
Wet Lab
28.07.09
29.07.09
30.07.09
31.07.09
August