"

 

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Revision as of 15:41, 29 July 2009 (view source) Bussingkm (Talk | contribs) (→Wetlab) ← Older edit		Revision as of 15:42, 29 July 2009 (view source) Bussingkm (Talk | contribs) (→Tasks) Newer edit →
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	* Transform J23110 with J06702 and pSB4C5		* Transform J23110 with J06702 and pSB4C5
	[[User:Trentay|Trentay]] 16:58, 28 July 2009 (UTC)		[[User:Trentay|Trentay]] 16:58, 28 July 2009 (UTC)
		+	''Kevin & Adam''
		+	*Continue research for next round of promoter synthesis
	----		----
	====Wetlab====		====Wetlab====

---

Revision as of 15:42, 29 July 2009

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Contents 1 Tuesday 28 July 2009 1.1 Results 1.2 Tasks 1.2.1 Wetlab

Tuesday 28 July 2009

Results

Maria and Afton

• Overnight Cultures were successful
  • J23110 w/ 702 was a yellow/pink color today
  • J23110 was very pink/red
  • J06702 and pSB4C5 grew

Trentay 16:58, 28 July 2009 (UTC)

---

Tasks

Maria and Afton

• Make Cryogenic stocks of J23110, J06702, pSB4C5, J23110 w/ 702
• Miniprep J23110, J06702, pSB4C5
• Digest and ligate J23110, J06702, pSB4C5
• Transform J23110 with J06702 and pSB4C5

Trentay 16:58, 28 July 2009 (UTC) Kevin & Adam

• Continue research for next round of promoter synthesis

---

Wetlab

Maria and Afton

• Cryogenic stocks were made
• Miniprep was done
• Digestion was done
• Ligation was done
• Transformation done

Trentay 18:15, 28 July 2009 (UTC) Kevin & Adam

• Transformed and plated ligated synthetic promoter assemblies

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