From 2009.igem.org
Tuesday 28 July 2009
Results
Maria and Afton
- Overnight Cultures were successful
- J23110 w/ 702 was a yellow/pink color today
- J23110 was very pink/red
- J06702 and pSB4C5 grew
- Miniprep/Spectrophotometry Results
| Part No. | A260 (nm) | A280 (nm) | A260/A280 | Vol. (µL) | Conc. (µg/mL) | Amt (µg)
|
| pSB4C5 | 0.142 | 0.085 | 1.67 | 37 | 284 | 10.50
|
| J23110 | 0.017 | 0.009 | 1.89 | 36 | 34 | 1.220
|
| J06702 | 0.009 | 0.005 | 1.80 | 32 | 18 | 0.576
|
Trentay 16:58, 28 July 2009 (UTC)
Tasks
Maria and Afton
- Make Cryogenic stocks of J23110, J06702, pSB4C5, J23110 w/ 702
- Miniprep J23110, J06702, pSB4C5
- Digest and ligate J23110, J06702, pSB4C5
- Transform J23110 with J06702 and pSB4C5
Trentay 16:58, 28 July 2009 (UTC)
Kevin & Adam
- Continue research for next round of promoter synthesis
Wetlab
Maria and Afton
- Cryogenic stocks were made
- Miniprep was done
- Digestion was done
- Ligation was done
- Transformation done
Trentay 18:15, 28 July 2009 (UTC)
Kevin & Adam
- Transformed and plated ligated synthetic promoter assemblies
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