Team:Newcastle/Labwork/31 July 2009

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(New page: {{:Team:Newcastle/CSS}} {{:Team:Newcastle/Header}} {{:Team:Newcastle/Left}} =Lab Session 31/07/2009= ==Previously== cinR ([http://partsregistry.org/Part:BBa_C0077 BBa_C0077]) and cinI (...)
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==Previously==
==Previously==
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cinR ([http://partsregistry.org/Part:BBa_C0077 BBa_C0077]) and cinI ([http://partsregistry.org/Part:BBa_C0076 BBa_C0076]) coding sequences did not grow in LB + Kan media but they grew in plain LB media. We had overnight cultures prepared for CinR sensitive promoter([http://partsregistry.org/Part:BBa_R0077 BBa_R0077]), cI coding sequence([http://partsregistry.org/Part:BBa_B1002 BBa_B1002]) and the double terminator([http://partsregistry.org/Part:BBa_B1002 BBa_B1002]).
+
''cinR'' ([http://partsregistry.org/Part:BBa_C0077 BBa_C0077]) and ''cinI'' ([http://partsregistry.org/Part:BBa_C0076 BBa_C0076]) coding sequences did not grow in LB + Kan media but they grew in plain LB media. We had overnight cultures prepared for CinR sensitive promoter([http://partsregistry.org/Part:BBa_R0077 BBa_R0077]), ''cI'' coding sequence([http://partsregistry.org/Part:BBa_B1002 BBa_B1002]) and the double terminator([http://partsregistry.org/Part:BBa_B1002 BBa_B1002]).
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 +
==Today's Aims==
 +
We will do a midi prep for the overnight cultures. We will follow the protocol from GenElute ([http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Bulletin/na0200bul.Par.0001.File.tmp/na0200bul.pdf NA0200S_NA0200]). The list of plasmid kits can be accessed from [http://www.sigmaaldrich.com/life-science/molecular-biology/dna-and-rna-purification/hp-plasmid-kits.html Gen Elute's page].

Revision as of 16:38, 2 August 2009

Lab Session 31/07/2009

Previously

cinR ([http://partsregistry.org/Part:BBa_C0077 BBa_C0077]) and cinI ([http://partsregistry.org/Part:BBa_C0076 BBa_C0076]) coding sequences did not grow in LB + Kan media but they grew in plain LB media. We had overnight cultures prepared for CinR sensitive promoter([http://partsregistry.org/Part:BBa_R0077 BBa_R0077]), cI coding sequence([http://partsregistry.org/Part:BBa_B1002 BBa_B1002]) and the double terminator([http://partsregistry.org/Part:BBa_B1002 BBa_B1002]).

Today's Aims

We will do a midi prep for the overnight cultures. We will follow the protocol from GenElute ([http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Bulletin/na0200bul.Par.0001.File.tmp/na0200bul.pdf NA0200S_NA0200]). The list of plasmid kits can be accessed from [http://www.sigmaaldrich.com/life-science/molecular-biology/dna-and-rna-purification/hp-plasmid-kits.html Gen Elute's page].





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