"
Team:Newcastle/Labwork/31 July 2009
From 2009.igem.org
(→Lab Session 31/07/2009) |
(→Today's Aims) |
||
| Line 9: | Line 9: | ||
==Today's Aims== | ==Today's Aims== | ||
| - | We will do a midi prep for the overnight cultures. We will follow the protocol from GenElute ([http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Bulletin/na0200bul.Par.0001.File.tmp/na0200bul.pdf NA0200S_NA0200]). The list of plasmid kits can be accessed from [http://www.sigmaaldrich.com/life-science/molecular-biology/dna-and-rna-purification/hp-plasmid-kits.html Gen Elute's page]. | + | We will do a midi prep for the overnight cultures. We will follow the protocol from GenElute ([http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Bulletin/na0200bul.Par.0001.File.tmp/na0200bul.pdf NA0200S_NA0200]). The list of plasmid kits can be accessed from [http://www.sigmaaldrich.com/life-science/molecular-biology/dna-and-rna-purification/hp-plasmid-kits.html Gen Elute's plasmid kits page]. |
Revision as of 16:38, 2 August 2009
Project
Sub-projects
Wet Lab
Meetings
Planning
Links
Lab Session 31/07/2009
Previously
cinR ([http://partsregistry.org/Part:BBa_C0077 BBa_C0077]) and cinI ([http://partsregistry.org/Part:BBa_C0076 BBa_C0076]) coding sequences did not grow in LB + Kan media but they grew in plain LB media. We had overnight cultures prepared for CinR sensitive promoter([http://partsregistry.org/Part:BBa_R0077 BBa_R0077]), cI coding sequence([http://partsregistry.org/Part:BBa_B1002 BBa_B1002]) and the double terminator([http://partsregistry.org/Part:BBa_B1002 BBa_B1002]).
Today's Aims
We will do a midi prep for the overnight cultures. We will follow the protocol from GenElute ([http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Bulletin/na0200bul.Par.0001.File.tmp/na0200bul.pdf NA0200S_NA0200]). The list of plasmid kits can be accessed from [http://www.sigmaaldrich.com/life-science/molecular-biology/dna-and-rna-purification/hp-plasmid-kits.html Gen Elute's plasmid kits page].
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]
