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Team:Warsaw/Calendar-Main/23 July 2009
From 2009.igem.org
(Difference between revisions)
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<li>Add 3.5 ml of the medium to the probes</li> | <li>Add 3.5 ml of the medium to the probes</li> | ||
<li>Add one bacterial colony to each probe</li> | <li>Add one bacterial colony to each probe</li> | ||
| - | <li>Breed the bacteria about 7 hours</li | + | <li>Breed the bacteria about 7 hours</li> |
| + | <h3><div style="text-align: center;">Construction of <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177012 ">K177012</a> operon1_part2 </div></h3> | ||
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| + | <h4>Ania</h4> | ||
| + | <br/> | ||
| + | <p>Tasks:</p> | ||
| + | |||
| + | <ul> | ||
| + | <li>Transformation of the chemocompetent cells with the ligation of <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_B0032"> BBa_B0032</a>- RBS.3 on the pSB1A2 ampicillin resistant plasmid and <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_C0012 BBa_C0012"> - lacI repressor</a> </li> | ||
</html> | </html> | ||
Revision as of 12:16, 3 August 2009
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PCR of Cox mitochondrial export sequence
Sebastian
Task 1:
- PCR with gradient temperature of anniling
Methods:
- Reaction mixture: 1ul pAcGFP1-Mito (5ng), 1ul dNTPs (2mM), 1ul L-primer (5pmol/ul), 1ul R-primer (5pmol/ul), 1ul Pfu buffer, 4,5ul ddH2o, 0,5ul Pfu DNA polymerase
- PCR program: 5min 95C, 1min 95C, 1min gradient 48-60C, 1min 72C, go to 2-nd step (30x), 5min 72C
Cloning of the mgtc promoter into the pKSII+ plasmid
Kamil
Tasks:
- Transformant selection
Methods:
- The colonies that remained white after 48h were picked up and transferred to a new petri dish.
- Liquid cultures were established along the way for plasmid purification.
- Both the dish and the liquid cultures were incubated at 37°C overnight.
Assembly of endosomal detection operon
Marcin
Task 1:
- Prepare the bacterial cultures for plasmid isolation
Preparation of bacterial cultures
Construction of K177012 operon1_part2
Ania
Tasks:
- Transformation of the chemocompetent cells with the ligation of BBa_B0032- RBS.3 on the pSB1A2 ampicillin resistant plasmid and - lacI repressor
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