From 2009.igem.org
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- | WIKI CODING HERE
| + | Researched on the reaction rates of various reactions, such as the binding affinity of AI-2 to LuxP. |
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Revision as of 22:06, 5 August 2009
University of Calgary
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CAROL
Mutagenesis Primers Set Up
Purpose: To set up primers at the correct concentration to perform the QuikChange XL Site-Directed Mutagenesis.
- Primers have to be set up to have a concentration of 125 ng/µL. The primers were ordered from the synthesis lab at the University of Calgary
The following are the concentrations:
Primer | 260/280 | 260/230 | Concentration [ng/μL] | Reading/2.5 [ng/μL]
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''luxCDABE-M-F | 1.52 | 2.33 | 409.5 | 163.8
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''luxCDABE-M-R | 1.58 | 2.86 | 479.8 | 191.2
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- Checked if we have NZY+ broth prepared (found in freezer).
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CHINMOYEE
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EMILY
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FAHD
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IMAN
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JAMIE
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JEREMY
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KATIE
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KEVIN
Modelling
Researched on the reaction rates of various reactions, such as the binding affinity of AI-2 to LuxP.
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MANDY
Descriptive Title of What You're Doing
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PATRICK
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PRIMA
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STEFAN
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VICKI
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