Team:TUDelft/RiboKeyLock Generator
From 2009.igem.org
(Difference between revisions)
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<form name="RBSinput" action="" method="get"> | <form name="RBSinput" action="" method="get"> | ||
Enter RBS DNA sequence:<br> | Enter RBS DNA sequence:<br> | ||
- | <input type="text" name="RBSdna" size=40 value=""> example: TCACACAGGAAACC<br><br> | + | <input type="text" name="RBSdna" size=40 value=""> example: TCACACAGGAAACC<br> |
+ | or select a BioBrick RBS:<br> | ||
- | + | <select name="BioBrickSelect" onChange="document.RBSinput.RBSdna.value=document.RBSinput.BioBrickSelect.options[document.RBSinput.BioBrickSelect.selectedIndex].value"> | |
- | + | <option value=TTCACACAGGAAACC>BBa_B0029</option> | |
+ | <option value=AUUAAAGAGGAGAAA>BBa_B0030</option> | ||
+ | <option value=UCACACAGGAAACC>BBa_B0031</option> | ||
+ | <option value=UCACACAGGAAAG>BBa_B0032</option> | ||
+ | <option value=UCACACAGGAC>BBa_B0033</option> | ||
+ | <option value=AAAGAGGAGAAA>BBa_B0034</option> | ||
+ | <option value=ATTAAAGAGGAGAA>BBa_B0035 </option> | ||
+ | <option value=AAAGAGGGGAAA>BBa_B0064</option> | ||
+ | <option value=TCACACCACCC>BBa_B0074</option> | ||
+ | <option value=UUUCUCCUCUUUAAU>BBa_J44001</option> | ||
+ | <option value=AAAGAGGGGACA>BBa_J61100</option> | ||
+ | <option value=AAAGACAGGACC>BBa_J61101</option> | ||
+ | <option value=AAAGAUCCGAUG>BBa_J61102</option> | ||
+ | <option value=AAAGAUUAGACA>BBa_J61103</option> | ||
+ | <option value=AAAGAAGGGACA>BBa_J61104</option> | ||
+ | <option value=AAAGACAUGACG>BBa_J61105</option> | ||
+ | <option value=AAAGAUAGGAGA>BBa_J61106</option> | ||
+ | <option value=AAAGAAGAGACU>BBa_J61107</option> | ||
+ | <option value=AAAGACGAGAUA>BBa_J61108</option> | ||
+ | <option value=AAAGACUGGAGA>BBa_J61109</option> | ||
+ | <option value=AAAGAGGCGAAU>BBa_J61110</option> | ||
+ | <option value=AAAGAGGCGAUA>BBa_J61111</option> | ||
+ | <option value=AAAGAGGUGACA>BBa_J61112</option> | ||
+ | <option value=AAAGAGUGGAAA>BBa_J61113</option> | ||
+ | <option value=AAAGAUGAGAAG>BBa_J61114</option> | ||
+ | <option value=AAAGAAGGGAUA>BBa_J61115</option> | ||
+ | <option value=AAAGACAUGAGG>BBa_J61116</option> | ||
+ | <option value=AAAGACAUGAGU>BBa_J61117</option> | ||
+ | <option value=AAAGAGACGAAU>BBa_J61118</option> | ||
+ | <option value=AAAGAUUUGAUA>BBa_J61119</option> | ||
+ | <option value=AAAGACGCGAGA>BBa_J61120</option> | ||
+ | <option value=AAAGAGACGAGU>BBa_J61121</option> | ||
+ | <option value=AAAGAGAGGAGC>BBa_J61122</option> | ||
+ | <option value=AAAGAGAUGACU>BBa_J61123</option> | ||
+ | <option value=AAAGAGCCGACA>BBa_J61124</option> | ||
+ | <option value=AAAGAGCCGAGU>BBa_J61125</option> | ||
+ | <option value=AAAGAGGUGACU>BBa_J61126</option> | ||
+ | <option value=AAAGAGUGGAAC>BBa_J61127</option> | ||
+ | <option value=AAAGAUAGGACU>BBa_J61128</option> | ||
+ | <option value=AAAGAUUGGACG>BBa_J61129</option> | ||
+ | <option value=AAAGAAACGACA>BBa_J61130</option> | ||
+ | <option value=AAAGAACCGAAU>BBa_J61131</option> | ||
+ | <option value=AAAGACAGGAUU>BBa_J61132</option> | ||
+ | <option value=AAAGACCCGAGA>BBa_J61133</option> | ||
+ | <option value=AAAGACCGGAAA>BBa_J61134</option> | ||
+ | <option value=AAAGACCGGAGA>BBa_J61135</option> | ||
+ | <option value=AAAGAGCUGAGC>BBa_J61136</option> | ||
+ | <option value=AAAGAGUAGAUC>BBa_J61137</option> | ||
+ | <option value=AAAGAUAUGAAU>BBa_J61138</option> | ||
+ | <option value=AAAGAUUAGAGU>BBa_J61139</option> | ||
+ | <option value=CCCGCCGCCACCAUGGAG>BBa_J63003</option> | ||
+ | <option value=AUUAAAGAGGAGAAAUA>BBa_K082000</option> | ||
+ | <option value=UCACACAGGAAAGUA>BBa_K082001</option> | ||
+ | <option value=AAAGAGGAGAAAUA>BBa_K082002</option> | ||
+ | <option value=CCCGCCGCCACCAUGGAG>BBa_K165002</option> | ||
+ | </select> | ||
+ | |||
+ | <br><br> | ||
<!--<input type="submit" value="Generate Key and Lock" />--> | <!--<input type="submit" value="Generate Key and Lock" />--> | ||
<a href="#" onclick="calckeylock()" title=""><img src="https://static.igem.org/mediawiki/2009/7/7a/TUD-ribo-keylock-button.png" border="0"></a> | <a href="#" onclick="calckeylock()" title=""><img src="https://static.igem.org/mediawiki/2009/7/7a/TUD-ribo-keylock-button.png" border="0"></a> | ||
<br><br> | <br><br> | ||
+ | |||
+ | RBS RNA sequence:<br> | ||
+ | <input type="text" name="RBS" size=40 value="" readonly="readonly"> example: UCACACAGGAAACC<br><br> | ||
+ | |||
Key RBS reverse complement RNA sequence with two mismatches:<br> | Key RBS reverse complement RNA sequence with two mismatches:<br> | ||
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Lock RBS reverse complement RNA sequence with one mismatch:<br> | Lock RBS reverse complement RNA sequence with one mismatch:<br> | ||
<input type="text" name="LOCKrevcommisRBS" size=40 value="" readonly="readonly"><br><br> | <input type="text" name="LOCKrevcommisRBS" size=40 value="" readonly="readonly"><br><br> | ||
+ | |||
+ | <hr><br> | ||
+ | Key RNA sequence:<br> | ||
+ | <textarea rows="5" cols="80" name="genkey" wrap="soft"> | ||
+ | </textarea> | ||
+ | <p> | ||
+ | Lock RNA sequence:<br> | ||
+ | <textarea rows="5" cols="80" name="genlock" wrap="soft"> | ||
+ | </textarea> | ||
+ | <p> | ||
+ | <a href=http://mfold.bioinfo.rpi.edu/cgi-bin/rna-form1.cgi>Jump to mfold</a> | ||
+ | <br><br> | ||
+ | <hr><br> | ||
+ | Key DNA sequence:<br> | ||
+ | <textarea rows="5" cols="80" name="genkeyDNA" wrap="soft"> | ||
+ | </textarea> | ||
+ | <p> | ||
+ | Lock DNA sequence:<br> | ||
+ | <textarea rows="5" cols="80" name="genlockDNA" wrap="soft"> | ||
+ | </textarea> | ||
+ | <br><br> | ||
<hr><br> | <hr><br> | ||
- | <b>Key | + | <b>Key variables:</b><p> |
Upstream<br> | Upstream<br> | ||
<input type="text" name="KEYupsteam" value="ACCCAAAGUCC" readonly="readonly" size=40><p> | <input type="text" name="KEYupsteam" value="ACCCAAAGUCC" readonly="readonly" size=40><p> | ||
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<hr><br> | <hr><br> | ||
- | <b>Lock | + | <b>Lock variables:</b><p> |
Upstream<br> | Upstream<br> | ||
<input type="text" name="LOCKupsteam" value="UUUGGGUAGAUCAC" readonly="readonly" size=40><p> | <input type="text" name="LOCKupsteam" value="UUUGGGUAGAUCAC" readonly="readonly" size=40><p> | ||
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<input type="text" name="LOCKposmis" value="3" readonly="readonly" size=40> | <input type="text" name="LOCKposmis" value="3" readonly="readonly" size=40> | ||
<br><br> | <br><br> | ||
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</form> | </form> |
Revision as of 08:19, 8 August 2009
Ribo Key & Lock Generator