Virginia Commonwealth/11 August 2009
From 2009.igem.org
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* Transform parts into NEB10β | * Transform parts into NEB10β | ||
* Update Wiki | * Update Wiki | ||
+ | * Make overnight cultures of I1352/I1352, I1352/pSB1C3 | ||
+ | * Make overnight cultures of pSB1C3 p1010 from cryogenic storage to Miniprep tomorrow | ||
[[User:Trentay|Trentay]] 14:43, 11 August 2009 (UTC) | [[User:Trentay|Trentay]] 14:43, 11 August 2009 (UTC) | ||
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Revision as of 14:50, 11 August 2009
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Contents |
Tuesday 11 August 2009
Results
Maria and Afton
- 1kb ladder has not come in yet so, digests and minipreps will be run on gels when it comes in
- Plated colonies from Friday (8/7/09) transformations are strongly espressing RFP
- It might be better to pick colonies 3 to 4 days after transformation when colonies are strongly espressing RFP, instead of when the e. coli are pink the day after transformation
Tasks
Maria and Afton
- Take Spectrophotometry measurements of pSB1C3 p1010, J06702, J23100, J23102, J23103, J23104
- Need to locate J23100 to be digested another day
- Digest above parts
- Ligate J06702 and pSB1C3 with the following promoters: 100, 102, 103, 104
- Transform parts into NEB10β
- Update Wiki
- Make overnight cultures of I1352/I1352, I1352/pSB1C3
- Make overnight cultures of pSB1C3 p1010 from cryogenic storage to Miniprep tomorrow
Trentay 14:43, 11 August 2009 (UTC)
Wetlab
Maria and Afton
- Spectrophotometry was done
- Digest was done
Trentay 14:43, 11 August 2009 (UTC)