Team:Newcastle/Labwork/17 August 2009

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==<u>Sporulation Tuning/ Chassis Team</u>==
==<u>Sporulation Tuning/ Chassis Team</u>==
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Today, we plan to repeat the experiment which we did on the [https://2009.igem.org/wiki/index.php?title=Team:Newcastle/Labwork/12_August_2009&action=edit&section=3 Wednesday,12th of August], which is the recovery of the cwlD spores.
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We plan to follow the protocol for [https://2009.igem.org/Team:Newcastle/Project/Labwork/MoreProtocols#Recovery_of_cwlD_spores Method A] again, however, this time, keeping in mind to add 40ul of our stock lysozyme instead of just 4ul.
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We will carry out the usual treatment of the spores with lysozyme, buffer solution and L-alanine. However, we will also attempt one extra "treatment", which is adding buffer solution to the spores and carrying out the procedure as usual, and lastly adding L-alanine. What we are trying to achieve here, is how great an effect lysozyme has on the spores.
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Revision as of 03:38, 16 August 2009

Lab 17/08/09

Sporulation Tuning/ Chassis Team

Today, we plan to repeat the experiment which we did on the Wednesday,12th of August, which is the recovery of the cwlD spores.

We plan to follow the protocol for Method A again, however, this time, keeping in mind to add 40ul of our stock lysozyme instead of just 4ul.

We will carry out the usual treatment of the spores with lysozyme, buffer solution and L-alanine. However, we will also attempt one extra "treatment", which is adding buffer solution to the spores and carrying out the procedure as usual, and lastly adding L-alanine. What we are trying to achieve here, is how great an effect lysozyme has on the spores.



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