Virginia Commonwealth/16 August 2009
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* 10µ of NEB10β seed stock was placed in 3 test tubes with 5mL of LB | * 10µ of NEB10β seed stock was placed in 3 test tubes with 5mL of LB | ||
** Two tubes are for the electrocompetency protocol, the third tube is for cryogenic stock | ** Two tubes are for the electrocompetency protocol, the third tube is for cryogenic stock | ||
* 10µ of J23113, J23116, R0011 were placed in test tubes with 5mL of LB+Amp | * 10µ of J23113, J23116, R0011 were placed in test tubes with 5mL of LB+Amp | ||
[[User:Trentay|Trentay]] 23:15, 17 August 2009 (UTC) | [[User:Trentay|Trentay]] 23:15, 17 August 2009 (UTC) | ||
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Latest revision as of 23:15, 17 August 2009
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Contents |
Sunday 16 August 2009
Results
- n/a
Tasks
Maria and Afton
- Make overnight cultures of NEB10β seed stock in preparation for making more electrocompetent cells
- Make overnight clutures of J23113, J23116, R0011 for Miniprep Monday
Trentay 23:15, 17 August 2009 (UTC)
Wetlab
Maria and Afton
- 10µ of NEB10β seed stock was placed in 3 test tubes with 5mL of LB
- Two tubes are for the electrocompetency protocol, the third tube is for cryogenic stock
- 10µ of J23113, J23116, R0011 were placed in test tubes with 5mL of LB+Amp
Trentay 23:15, 17 August 2009 (UTC)