"

 

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Revision as of 14:13, 18 August 2009 (view source) Bussingkm (Talk | contribs) (→Tuesday 18 August 2009) ← Older edit		Revision as of 14:40, 18 August 2009 (view source) Trentay (Talk | contribs) (→Tuesday 18 August 2009) Newer edit →
Line 39:		Line 39:
	==Tuesday 18 August 2009==		==Tuesday 18 August 2009==
	===Results===		===Results===
-	* 1	+	''Maria and Afton''
-	* 2	+	* Re-Inoculated cultures with 10µ sample in 5mL of LB+Amp show no expression
		+	** Overnight cultures made Sunday: only J23116 is expressing florescence (slightly pink)
		+	* Electrocompetency was completed
		+	** 80 vials of 250µL Electrocompetent NEB10β was made
		+	** They will be tested today to ensure the procedure was successful
		+	[[User:Trentay|Trentay]] 14:11, 18 August 2009 (UTC)
	----		----
		+
	===Tasks===		===Tasks===
-	* 1 Kevin- PCR	+	''Kevin''
-	* 2	+	* PCR
		+
		+	''Maria and Afton''
		+	* Do Spectrophotometry of E0240 and pSB1C3
		+	* Digest E0240 and pSB1C3 w/p1010
		+	* Run a gel on E0240 and pSB1C3 w/p1010
		+	* Ligate E0240 w/ pSB1C3 with J23100-104
		+	** Digests from last week will be used for ligation
		+	** However, I would prefer to transform Ligations with J06702 and see how well those grow before proceeding to further ligations
		+
		+	* Transform Ligations with pSB1C3 and J06702 with: J23100-J23107, R0011, R0040
		+	* Transform newly made electrocompetent cells to make sure they are working
		+	[[User:Trentay|Trentay]] 14:20, 18 August 2009 (UTC)
	----		----
	====Wetlab====		====Wetlab====
		+	''Maria and Afton''
	* 1		* 1
	* 2		* 2
		+	[[User:Trentay|Trentay]] 14:20, 18 August 2009 (UTC)

---

Revision as of 14:40, 18 August 2009

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October M T W T F S S       [http://2009.igem.org/Virginia_Commonwealth/1_October_2009 1] [http://2009.igem.org/Virginia_Commonwealth/2_October_2009 2] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/3_October_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_October_2009&action=edit 4] [http://2009.igem.org/Virginia_Commonwealth/5_October_2009 5] [http://2009.igem.org/Virginia_Commonwealth/6_October_2009 6] [http://2009.igem.org/Virginia_Commonwealth/7_October_2009 7] [http://2009.igem.org/Virginia_Commonwealth/8_October_2009 8] [http://2009.igem.org/Virginia_Commonwealth/9_October_2009 9] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/10_October_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_October_2009&action=edit 11] [http://2009.igem.org/Virginia_Commonwealth/12_October_2009 12] [http://2009.igem.org/Virginia_Commonwealth/13_October_2009 13] [http://2009.igem.org/Virginia_Commonwealth/14_October_2009 14] [http://2009.igem.org/Virginia_Commonwealth/15_October_2009 15] [http://2009.igem.org/Virginia_Commonwealth/16_October_2009 16] [http://2009.igem.org/Virginia_Commonwealth/17_October_2009 17] [http://2009.igem.org/Virginia_Commonwealth/18_October_2009 18] [http://2009.igem.org/Virginia_Commonwealth/19_October_2009 19] [http://2009.igem.org/Virginia_Commonwealth/20_October_2009 20] [http://2009.igem.org/Virginia_Commonwealth/21_October_2009 21] [http://2009.igem.org/Virginia_Commonwealth/22_October_2009 22] [http://2009.igem.org/Virginia_Commonwealth/23_October_2009 23] [http://2009.igem.org/Virginia_Commonwealth/24_October_2009 24] [http://2009.igem.org/Virginia_Commonwealth/25_October_2009 25] [http://2009.igem.org/Virginia_Commonwealth/26_October_2009 26] [http://2009.igem.org/Virginia_Commonwealth/27_October_2009 27] [http://2009.igem.org/Virginia_Commonwealth/28_October_2009 28] [http://2009.igem.org/Virginia_Commonwealth/29_October_2009 29] [http://2009.igem.org/Virginia_Commonwealth/30_October_2009 30] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/31_October_2009&action=edit 31]

Contents 1 Tuesday 18 August 2009 1.1 Results 1.2 Tasks 1.2.1 Wetlab

Tuesday 18 August 2009

Results

Maria and Afton

• Re-Inoculated cultures with 10µ sample in 5mL of LB+Amp show no expression
  • Overnight cultures made Sunday: only J23116 is expressing florescence (slightly pink)
• Electrocompetency was completed
  • 80 vials of 250µL Electrocompetent NEB10β was made
  • They will be tested today to ensure the procedure was successful

Trentay 14:11, 18 August 2009 (UTC)

---

Tasks

Kevin

• PCR

Maria and Afton

• Do Spectrophotometry of E0240 and pSB1C3
• Digest E0240 and pSB1C3 w/p1010
• Run a gel on E0240 and pSB1C3 w/p1010
• Ligate E0240 w/ pSB1C3 with J23100-104
  • Digests from last week will be used for ligation
  • However, I would prefer to transform Ligations with J06702 and see how well those grow before proceeding to further ligations

• Transform Ligations with pSB1C3 and J06702 with: J23100-J23107, R0011, R0040
• Transform newly made electrocompetent cells to make sure they are working

Trentay 14:20, 18 August 2009 (UTC)

---

Wetlab

Maria and Afton

• 1
• 2

Trentay 14:20, 18 August 2009 (UTC)

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