Team:Newcastle/Project/Labwork/OurProtocols/Aspirator

From 2009.igem.org

(Difference between revisions)
(New page: {{:Team:Newcastle/CSS}} {{:Team:Newcastle/Header}} {{:Team:Newcastle/Left}} __NOTOC__ =Using the Aspirator= * Make sure the valve of the aspirator is set to the closed position; if in the ...)
(→Using the Aspirator)
Line 7: Line 7:
* Switch on the cooler.
* Switch on the cooler.
* Set 'Drying Rate' to "HIGH"
* Set 'Drying Rate' to "HIGH"
-
 
+
<br>
It is important that the pump and the cooler have been running for at least 10 minutes prior to carrying out aspirations - this gives the machine time to reach the correct temperature.
It is important that the pump and the cooler have been running for at least 10 minutes prior to carrying out aspirations - this gives the machine time to reach the correct temperature.
-
 
+
<br>
* Open the aspirator lid and insert the Eppendorf tubes making sure that the lids are open. Also ensure that the Eppendorf tubes are placed in a symmetrical fashion so that a balance is maintained.
* Open the aspirator lid and insert the Eppendorf tubes making sure that the lids are open. Also ensure that the Eppendorf tubes are placed in a symmetrical fashion so that a balance is maintained.
* Set the 'Concentrator' to "ON"  
* Set the 'Concentrator' to "ON"  

Revision as of 10:49, 21 August 2009

Using the Aspirator

  • Make sure the valve of the aspirator is set to the closed position; if in the closed position, switch on the pump.
  • Switch on the cooler.
  • Set 'Drying Rate' to "HIGH"


It is important that the pump and the cooler have been running for at least 10 minutes prior to carrying out aspirations - this gives the machine time to reach the correct temperature.

  • Open the aspirator lid and insert the Eppendorf tubes making sure that the lids are open. Also ensure that the Eppendorf tubes are placed in a symmetrical fashion so that a balance is maintained.
  • Set the 'Concentrator' to "ON"
  • Slowly set the valve to the open position.
  • Leave samples in the aspirator for 5 minutes initially, checking on it's progress afterwards.



News

Events

Social Net

  • Newcastle iGEM Twitter
  • [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
  • [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]
Retrieved from "http://2009.igem.org/Team:Newcastle/Project/Labwork/OurProtocols/Aspirator"