Team:UQ-Australia/Notebook

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=='''Bioprecipitation Project'''==
=='''Bioprecipitation Project'''==
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'''11/08/09'''
'''11/08/09'''
No results were given from the transformation. Transformation will have to be repeated.
No results were given from the transformation. Transformation will have to be repeated.

Revision as of 13:08, 24 August 2009

Water Purification Project

July
MTWTFSS
    [http://2009.igem.org/wiki/index.php?title=UQ-Australia/1_July_2009&action=edit 1] [http://2009.igem.org/UQ-Australia/2_July_2009 2] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/3_July_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/5_July_2009&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=UQ-Australia/6_July_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/7_July_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/8_July_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/9_July_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/10_July_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/12_July_2009&action=edit 12]
[http://2009.igem.org/wiki/index.php?title=UQ-Australia/13_July_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/14_July_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/15_July_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/16_July_2009&action=edit 16] [http://2009.igem.org/UQ-Australia/17_July_2009 17] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/19_July_2009&action=edit 19]
[http://2009.igem.org/wiki/index.php?title=UQ-Australia/20_July_2009&action=edit 20] [http://2009.igem.org/UQ-Australia/21_July_2009 21] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/22_July_2009&action=edit 22] [http://2009.igem.org/UQ-Australia/23_July_2009 23] [http://2009.igem.org/UQ-Australia/24_July_2009 24] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/25_July_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/26_July_2009&action=edit 26]
[http://2009.igem.org/UQ-Australia/27_July_2009 27] [http://2009.igem.org/UQ-Australia/28_July_2009 28] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/29_July_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/30_July_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/31_July_2009&action=edit 31]
August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=UQ-Australia/1_August_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/2_August_2009&action=edit 2]
[http://2009.igem.org/UQ-Australia/3_August_2009 3] [http://2009.igem.org/UQ-Australia/4_August_2009 4] [http://2009.igem.org/UQ-Australia/5_August_2009 5] [http://2009.igem.org/UQ-Australia/6_August_2009 6] [http://2009.igem.org/UQ-Australia/7_August_2009 7] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/8_August_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/9_August_2009&action=edit 9]
[http://2009.igem.org/UQ-Australia/10_August_2009 10] [http://2009.igem.org/UQ-Australia/11_August_2009 11] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/12_August_2009&action=edit 12] [http://2009.igem.org/UQ-Australia/13_August_2009 13] [http://2009.igem.org/UQ-Australia/14_August_2009 14] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/15_August_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/16_August_2009&action=edit 16]
[http://2009.igem.org/UQ-Australia/17_August_2009 17] [http://2009.igem.org/UQ-Australia/18_August_2009 18] [http://2009.igem.org/UQ-Australia/19_August_2009 19] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/20_August_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/21_August_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/22_August_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/23_August_2009&action=edit 23]
[http://2009.igem.org/UQ-Australia/24_August_2009 24] [http://2009.igem.org/UQ-Australia/25_August_2009 25] [http://2009.igem.org/UQ-Australia/26_August_2009 26] [http://2009.igem.org/UQ-Australia/27_August_2009 27] [http://2009.igem.org/UQ-Australia/28_August_2009 28] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/29_August_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/30_August_2009&action=edit 30]
[http://2009.igem.org/wiki/index.php?title=UQ-Australia/31_August_2009&action=edit 31]

24/07/09 - DNA extraction and electrophoresis
Mini prep DNA extractions performed for MS427-pBAD, MS427-pKKJ143 cultures incubated overnight.
--> run on ethidium bromide gel to confirm DNA production.
Click HERE for a picture of the gel.

23/07/09 - Transformations with AG43 plasmids
E. coli incubated overnight in duplicate under the following conditions:

Strain: MS427 

Plasmid: pBAD (empty plasmid -AG43) 

- 2mL LB broth 

- 2µL ampicillin 




Strain: MS427 

Plasmid: pKKJ143 (plasmid w/ AG43) 

- 2mL LB broth 

- 2µL ampicillin

17/07/09 - MG1655 Stock Preparation
MG1655 E. coli grown on pure LB stock. No growth was observed using LB + ampicillin medium.
Pelleted and resuspended in 50:50 glycerol:LB medium and stored at -80 degrees C.

Bioprecipitation Project

August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=UQ-Australia/1_August_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/2_August_2009&action=edit 2]
[http://2009.igem.org/UQ-Australia/3_August_2009 3] [http://2009.igem.org/UQ-Australia/4_August_2009 4] [http://2009.igem.org/UQ-Australia/5_August_2009 5] [http://2009.igem.org/UQ-Australia/6_August_2009 6] [http://2009.igem.org/UQ-Australia/7_August_2009 7] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/8_August_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/9_August_2009&action=edit 9]
[http://2009.igem.org/UQ-Australia/10_August_2009 10] [http://2009.igem.org/UQ-Australia/11_August_2009 11] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/12_August_2009&action=edit 12] [http://2009.igem.org/UQ-Australia/13_August_2009 13] [http://2009.igem.org/UQ-Australia/14_August_2009 14] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/15_August_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/16_August_2009&action=edit 16]
[http://2009.igem.org/UQ-Australia/17_August_2009 17] [http://2009.igem.org/UQ-Australia/18_August_2009 18] [http://2009.igem.org/UQ-Australia/19_August_2009 19] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/20_August_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/21_August_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/22_August_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/23_August_2009&action=edit 23]
[http://2009.igem.org/UQ-Australia/24_August_2009 24] [http://2009.igem.org/UQ-Australia/25_August_2009 25] [http://2009.igem.org/UQ-Australia/26_August_2009 26] [http://2009.igem.org/UQ-Australia/27_August_2009 27] [http://2009.igem.org/UQ-Australia/28_August_2009 28] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/29_August_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/30_August_2009&action=edit 30]
[http://2009.igem.org/wiki/index.php?title=UQ-Australia/31_August_2009&action=edit 31]
September
MTWTFSS
  [http://2009.igem.org/UQ-Australia/1_September_2009 1] [http://2009.igem.org/UQ-Australia/2_September_2009 2] [http://2009.igem.org/UQ-Australia/3_September_2009 3] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/4_September_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/5_September_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/6_September_2009&action=edit 6]
[http://2009.igem.org/UQ-Australia/7_September_2009 7] [http://2009.igem.org/UQ-Australia/8_September_2009 8] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/9_September_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/10_September_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/11_September_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/12_September_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/13_September_2009&action=edit 13]
[http://2009.igem.org/UQ-Australia/14_September_2009 14] [http://2009.igem.org/UQ-Australia/15_September_2009 15] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/16_September_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/17_September_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/18_September_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/19_September_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/20_September_2009&action=edit 20]
[http://2009.igem.org/wiki/index.php?title=UQ-Australia/21_September_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/22_September_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/23_September_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/24_September_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/25_September_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/26_September_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/27_September_2009&action=edit 27]
[http://2009.igem.org/wiki/index.php?title=UQ-Australia/28_September_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/29_September_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/30_September_2009&action=edit 30]

11/08/09 No results were given from the transformation. Transformation will have to be repeated.


10/08/09 Transformation of our 4 plasmids took place. They were transformed into TOP10 bacteria. A colony check will be preformed tomorrow morning.

To see protocol, click HERE


7/08/09 Vectors were examined and we are planning for the best cloning strategy for the standard plasmids.


6/08/09 Plasmid solutions were left overnight and in the morning checked again with Nanodrop. No DNA was measured. Samples were put in the waterbath at 37 degrees and left for a couple of hours. If Nanodrop does not work after the waterbath we will still do transformation since we only need few plasmids for the transformation to work. Nanodrop may not have been able to quantify such a small concentration of plasmids.

LB media was poured on petri dishes and plasmid DNA was checked again using Nanodrop.

  • pXCK-EL = 10.55 ng/uL
  • pXCK-ES = 8.43 ng/uL
  • pXCK-K = 11.73 ng/uL
  • pXCK-E/J = 2.66 ng/uL

Plasmids were stored and transformation will be done on Monday.


5/08/09

Plasmids have arrived!

We have put them into 150 mL of TE buffer and left overnight for the paper to dissolve and later extract the plasmid from the solution.

A Nanodrop was used to see if the DNA was coming out of the paper. No DNA was detected, thus samples were left overnight.

Click on the plasmid name to look at the vector

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