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Template:Team:KULeuven/24 August 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
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| - | # | + | # plates with ligA were put under blue light. the LEDs were put on their max capacity. |
# restriction digest with | # restriction digest with | ||
#*tubes (1,3,5,7,9) of ligA (BLP + E0240) cut with EcoRI en PstI | #*tubes (1,3,5,7,9) of ligA (BLP + E0240) cut with EcoRI en PstI | ||
Revision as of 09:35, 27 August 2009
- plates with ligA were put under blue light. the LEDs were put on their max capacity.
- restriction digest with
- tubes (1,3,5,7,9) of ligA (BLP + E0240) cut with EcoRI en PstI
- promotor J23101 cut with EcoRI en SpeI (4x)
- gel electroforese with the RD of ligA and J23101 followed by a gel extraction:
- note: tube 5 of ligA was loaded poorly on the gel and could not be used.
- note: the samples of promotor J23101 were barly visible. only 2 of the 4 samples were recoverd by extraction.
| Part | concentration (ng/μl) | 260/280 λ | |
|---|---|---|---|
| ligA 1 | 7,9 | 2,16 | |
| ligA 3 | 10,8 | 1,88 | |
| ligA 7 | 4,8 | 2,49 | |
| ligA 9 | 8,0 | 3,02 | |
| (A) | 20,8 | 1,70 | |
| (B) | 12,8 | 2,57 |
4. enting of liquid cultures with kanamycin and pSB3K3
