Team:Newcastle/Labwork/26 August 2009

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=Lab Work - 26/08/09=
=Lab Work - 26/08/09=
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==<u>Stochastic Switch team</u>==
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===Summary===
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Today Lab started at 2pm, so we had the afternoon to do restriction digests for the remaining biobricks(other than C0178). We used the fast digest system, which takes 5 minutes in a 37 degree waterbath rather than 60 minutes. Below are the volumes required for a double digest using EcoRI and PstI:
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* 6ul water
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* 1ul of each Fast digest enzyme
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* 10ul DNA (miniprepped)
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* 2ul 10X Fast digest buffer#
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Total volume: 20ul
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 +
These were left for 5-10 minutes at 37 degrees, then 9ul of the total volume was added to 1ul of loading buffer, and the samples were run on the gel we prepared yesterday.
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Today we also prepared some overnight cultures of DH5alpha cells (2X 3ml plain LB) for the Genomic DNA prep which will be carried out tomorrow.
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==<u>Metal Sensing Team team</u>==
==<u>Metal Sensing Team team</u>==
{{:Team:Newcastle/Footer}}
{{:Team:Newcastle/Footer}}
{{:Team:Newcastle/Right}}
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Revision as of 11:07, 27 August 2009

Contents

Lab Work - 26/08/09

Stochastic Switch team

Summary

Today Lab started at 2pm, so we had the afternoon to do restriction digests for the remaining biobricks(other than C0178). We used the fast digest system, which takes 5 minutes in a 37 degree waterbath rather than 60 minutes. Below are the volumes required for a double digest using EcoRI and PstI:

  • 6ul water
  • 1ul of each Fast digest enzyme
  • 10ul DNA (miniprepped)
  • 2ul 10X Fast digest buffer#

Total volume: 20ul

These were left for 5-10 minutes at 37 degrees, then 9ul of the total volume was added to 1ul of loading buffer, and the samples were run on the gel we prepared yesterday.

Today we also prepared some overnight cultures of DH5alpha cells (2X 3ml plain LB) for the Genomic DNA prep which will be carried out tomorrow.


Metal Sensing Team team



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