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Uppsala-Sweden/31 August 2009
From 2009.igem.org
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==Adh2Y PCR== | ==Adh2Y PCR== | ||
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[[Image:20090831_Adh2Y-labelled.jpg]] | [[Image:20090831_Adh2Y-labelled.jpg]] | ||
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| + | Gel of Adh2Y PCR with BBa extension primers on Bakers Yeast lysate (Wine yeast did not work). | ||
--[[User:Karl.brune|Karl.brune]] 15:18, 1 September 2009 (UTC) | --[[User:Karl.brune|Karl.brune]] 15:18, 1 September 2009 (UTC) | ||
Revision as of 15:19, 1 September 2009
Adh2Y PCR
Gel of Adh2Y PCR with BBa extension primers on Bakers Yeast lysate (Wine yeast did not work). --Karl.brune 15:18, 1 September 2009 (UTC)
Transformation
Digestion of RBS and Adh2Y PCR product prior to ligation. Media:Digestion(excert)090831.xls
--Anders 13:20, 31 August 2009 (UTC)
Digestion and cleanup report, 100 times dilution: Media:digest_purification_measur_09831.txt
--Anders 15:11, 31 August 2009 (UTC)
Amounts to be used for Assembly: Media:Assembly volume calcuator090831_1.xls and Biobricking: Media:Ligation voulume calcuator_090831_1.xls
--Anders 16:11, 31 August 2009 (UTC)
