Team:EPF-Lausanne/Protocols/PCR
From 2009.igem.org
(Difference between revisions)
(→Cycles) |
(→Cycles) |
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:94°C – 120’’ | :94°C – 120’’ | ||
- | :94°C – 45’’ & | + | :94°C – 45’’ g |
:56°C – 45’’ → 30x | :56°C – 45’’ → 30x | ||
:68°C – 120’’ | :68°C – 120’’ |
Revision as of 07:23, 4 September 2009
Primers
Make primers pair mix with 3µM concentration (total).
Plasmid | 0.5ul |
---|---|
Primers pair (3µM) | 1ul |
Thermo POL buffer | 2.5µl |
dNTP | 0.5 µl |
Taq EPFL polym | 0.25 µl |
H20 (MQ) | 20.25 µl |
Total | 24.5 µl |
Cycles
Program PCR: count 1min/kb
- 94°C – 120’’
- 94°C – 45’’ g
- 56°C – 45’’ → 30x
- 68°C – 120’’
- 72°C – 420’'
- 10°C – ∞